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Review
. 2025 Jun 7;14(12):2020.
doi: 10.3390/foods14122020.

Unveiling the Impact of Processing Methods on In Vitro Protein Digestibility: A Focus on Highland Barley and Its Application in Wine Production

Mengchuan He  1 , Xinjing Zhou  1 , András Koris  2 , Bingyu Chen  1 , Xuchun Zhu  1 , Feiyue Ren  1 , Hongzhi Liu  1
Affiliations
Review

Unveiling the Impact of Processing Methods on In Vitro Protein Digestibility: A Focus on Highland Barley and Its Application in Wine Production

Mengchuan He et al. Foods. .

Abstract

As one of the indispensable components of the human body, proteins not only participate in the construction of tissues, but also play an important role in maintaining the metabolic balance of the organism. However, in some protein sources, factors such as complex protein structures and the presence of anti-nutritional compounds reduce bioavailability. Processing methods can significantly enhance protein digestibility by overcoming these limitations. This review focuses on highland barley proteins and their application in winemaking. It summarises their composition, digestibility and functional properties. It also summarises and evaluates in vitro digestion models suitable for in vitro digestion of highland barley proteins. Then, this review investigates in depth the effects of different processing methods-including thermal treatments, enzymatic hydrolysis, and microbial fermentation-on the in vitro digestibility of highland barley proteins and their mechanisms during winemaking. It was shown that these processing methods achieve different degrees of in vitro protein digestibility improvement by altering the structure of highland barley proteins and altering the interactions with other substances, achieving efficient utilisation of high-quality highland barley protein resources. Finally, this review provides a prospect which opens up new ideas for the efficient utilisation of proteins in food processing in the future.

Keywords: highland barley protein; highland barley wine; in vitro digestive model; in vitro protein digestibility; processing method.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
Biofunctional properties of highland barley protein.
Figure 2
Figure 2
Protocols used to evaluate digestibility in the context of processed highland barley. (a) Determination of enzyme activity and bile assay; (b) oral, gastric, and intestinal digestion simulation; (c) centrifugation of samples to obtain supernatant.
Figure 3
Figure 3
The unique role of highland barley in the production of highland barley wine.
Figure 4
Figure 4
The modern brewing process of highland barley wine.
Figure 5
Figure 5
Effect of processing methods of thermal processing, fermentation, and enzymatic treatment on protein structure and in vitro protein digestibility.
See this image and copyright information in PMC

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References

    1. Shi T., Cao J., Cao J., Zhu F., Cao F., Su E. Almond (Amygdalus communis L.) kernel protein: A review on the extraction, functional properties and nutritional value. Food Res. Int. 2023;167:11. doi: 10.1016/j.foodres.2023.112721. - DOI - PubMed
    1. Dolganyuk V., Sukhikh S., Kalashnikova O., Ivanova S., Kashirskikh E., Prosekov A., Michaud P., Babich O. Food Proteins: Potential Resources. Sustainability. 2023;15:5863. doi: 10.3390/su15075863. - DOI
    1. Rodriguez N.R. Introduction to Protein Summit 2.0: Continued exploration of the impact of high-quality protein on optimal health. Am. J. Clin. Nutr. 2023;101:1317S–1319S. doi: 10.3945/ajcn.114.083980. - DOI - PubMed
    1. Guo T., Horvath C., Chen L., Chen J., Zheng B. Understanding the nutrient composition and nutritional functions of highland barley (Qingke): A review. Trends Food Sci. Technol. 2020;103:109–117. doi: 10.1016/j.tifs.2020.07.011. - DOI
    1. Du Y., Liang F., Chen Z., Zhou W., Tu Z., Li J. Effects of decolorization on aggregation behavior of highland barley proteins: Comparison with wheat proteins. Food Res. Int. 2022;160:10. doi: 10.1016/j.foodres.2022.111712. - DOI - PubMed

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