Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2025 Jun 18;14(12):2131.
doi: 10.3390/foods14122131.

Preparation, Characterization, and In Vitro Stability Analysis of Deer Sinew Peptide-Zinc Chelate

Shan Yang  1 , Tianyuan Liu  1 , Weijia Chen  1 , Ying Zong  1 , Jianan Geng  1 , Yan Zhao  1 , Zhongmei He  1   2 , Rui Du  1   2
Affiliations

Preparation, Characterization, and In Vitro Stability Analysis of Deer Sinew Peptide-Zinc Chelate

Shan Yang et al. Foods. .

Abstract

Novel peptide-zinc chelates (DSPs-Zn) with a zinc content of 186.94 mg/g were synthesized from deer tendon peptides at pH 6, 60 °C, 60 min, and peptide-zinc mass ratio of 1:3. Ultraviolet-visible absorption spectroscopy (UV) and Fourier transform infrared spectroscopy (FTIR) demonstrated that the chelation sites of the deer tendon polypeptides (DSPs) with zinc ions were located at the carboxyl oxygen and amino nitrogen atoms of the peptides. Amino acid analysis showed that aspartic acid, glutamic acid, lysine, and arginine play important roles in the chelation process. In vitro simulated gastrointestinal digestion studies showed that DSPs-zinc exhibited higher stability than zinc sulfate and zinc gluconate in the pH range 2-8 and in a simulated gastrointestinal digestion environment. The above experimental results suggest that DSPs-Zn has the potential to be used as a novel zinc nutritional supplement.

Keywords: deer tendon protein; peptides-zinc chelate; response surface methodology; simulated gastrointestinal digestion; structural characterization.

PubMed Disclaimer

Conflict of interest statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Figure 1
Figure 1
Graphs illustrate the influence of pH (A), temperature (B), duration (C), and the mass ratio of DSPs to ZnSO4-7H2O (D) on the zinc binding capacity. Note: DSPs: deer tendon polypeptides; DSPs-Zn: deer tendon polypeptide zinc chelates.
Figure 2
Figure 2
Response surface plots of the effects of three factors on zinc content. (A) Interaction of A (pH value) and B (mass ratio of DSP: zinc). (B) Interaction of A (pH value) and C (temperature). (C) Interaction of B (mass ratio of DSP: zinc) and C (temperature).
Figure 3
Figure 3
(A) UV-vis spectra, (B) FTIR spectra, (C) particle size distribution, (D) zeta potential, (E) scanning electron microscopy of DSPs and (F) DSPs-Zn; (G) surface elemental composition of DSPs and (H) DSPs-Zn. Different letters indicate statistically significant differences between groups (p < 0.05).
Figure 4
Figure 4
Zinc solubility of DSPs-Zn at different pH (A) and simulated gastrointestinal digestion (B). Note: DSPs-Zn, deer gluten peptide zinc chelates. Different letters indicate statistically significant differences between groups (p < 0.05).
See this image and copyright information in PMC

Similar articles

See all similar articles

References

    1. Huang C., Hightower K.E., Fierke C.A. Mechanistic Studies of Rat Protein Farnesyltransferase Indicate an Associative Transition State. Biochemistry. 2000;39:2593–2602. doi: 10.1021/bi992356x. - DOI - PubMed
    1. Halsted C.H. Dietary Supplements and the American Journal of Clinical Nutrition. Am. J. Clin. Nutr. 2023;71:399–400. doi: 10.1093/ajcn/71.2.399. - DOI - PubMed
    1. Costa M.I., Sarmento-Ribeiro A.B., Gonçalves A.C. Zinc: From Biological Functions to Therapeutic Potential. Int. J. Mol. Sci. 2023;24:4822. doi: 10.3390/ijms24054822. - DOI - PMC - PubMed
    1. Hussain A., Jiang W., Wang X., Shahid S., Saba N., Ahmad M., Dar A., Masood S.U., Imran M., Mustafa A. Mechanistic Impact of Zinc Deficiency in Human Development. Front. Nutr. 2022;9:717064. doi: 10.3389/fnut.2022.717064. - DOI - PMC - PubMed
    1. Gregory P.J., Wahbi A., Adu-Gyamfi J., Heiling M., Gruber R., Joy E.J.M., Broadley M.R. Approaches to Reduce Zinc and Iron Deficits in Food Systems. Glob. Food Secur. 2017;15:1–10. doi: 10.1016/j.gfs.2017.03.003. - DOI

LinkOut - more resources