LINC00365 serves as a prognostic biomarker for thyroid cancer and regulates cell proliferation, migration and invasion by targeting miR-485-5p

Abstract

Thyroid cancer (TC) is the most prevalent form of malignancy within the endocrine system globally, and its incidence has been increasing significantly in recent years. The aim of this study was to explore the clinical role of LINC00365 in TC, and to analyze the regulatory mechanism between LINC00365 and miR-485-5p and its specific impact on TC. This study included 135 TC patients. The levels of LINC00365 in TC tissues and cells were detected by RT-qPCR. The impact of LINC00365 on TC prognosis was investigated using Kaplan-Meier curve and multivariate Cox regression analysis. Furthermore, the impact of LINC00365 on TC cell proliferation was examined via CCK-8 assay, while transwell assays were utilized to evaluate changes in migration and invasion capabilities. The regulatory interaction between LINC00365 and miR-485-5p was confirmed through dual-luciferase reporter assay. The expression of LINC00365 was upregulated in TC tissues and cells, and its high expression was closely related to the poor prognosis of TC patients. Kaplan-Meier curve revealed a notable correlation between elevated LINC00365 expression levels and reduced survival outcomes and multivariate Cox regression analysis revealed that elevated LINC00365 expression levels served as an independent prognostic indicator, significantly associated with adverse clinical outcomes in TC patients. In addition, LINC00365 negatively regulated the expression level of miR-485-5p. Inhibiting LINC00365 may suppress the proliferation, migration and invasion abilities of TC cells. LINC00365 may be a potential prognostic indicator for TC, and LINC00365 may promote tumor growth and metastasis of TC by inhibiting miR-485-5p expression.

Keywords: LINC00365; miR-485-5p; prognosis; thyroid cancer.