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. 2025 Jul 25;31(42):e202501721.
doi: 10.1002/chem.202501721. Epub 2025 Jul 7.

New Click on the Block: Photocaged Hydroxylamine, an Addition to the Light-Activatable Bioorthogonal Toolbox

Krisztina Németh  1 , Flóra Horváth  1 , Ágnes Szatmári  1 , Evelin Albitz  1 , Péter Kele  1 , Gergely B Cserép  1
Affiliations

New Click on the Block: Photocaged Hydroxylamine, an Addition to the Light-Activatable Bioorthogonal Toolbox

Krisztina Németh et al. Chemistry. .

Abstract

A new photoactivatable bioorthogonal function was added to the photoactivatable bioorthogonal toolbox by transiently disabling the bioorthogonal reactivity of the hydroxylamine moiety with a photolabile protecting group. The photocaged bioorthogonal function was further equipped with a HaloTag substrate to enable its use in live-cell labeling schemes. Light-controlled recovery of reactivity was demonstrated by bioorthogonal fluorescent labeling of intracellular proteins in live cells.

Keywords: bioorthogonal; caged hydroxylamine; light activation; photocage; retro Cope ligation.

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