Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2025:2943:157-166.
doi: 10.1007/978-1-0716-4642-7_13.

Power of Short Tandem Repeat (STR) Analysis for Analysis of Genetic Differences and Genotype Identification

Anthony Torres  1 , Reginald Gaudino  2   1
Affiliations

Power of Short Tandem Repeat (STR) Analysis for Analysis of Genetic Differences and Genotype Identification

Anthony Torres et al. Methods Mol Biol. 2025.

Abstract

This chapter presents a protocol for short tandem repeat (STR) analysis, a robust, economical, and highly accurate method commonly used in forensics and agriculture for genetic profiling and identity determination. The protocol involves DNA isolation from FTA cards or leaf pieces, followed by PCR amplification of STR regions and fragment analysis. The number of repeats in these short tandem patterns is unique to individuals, making it useful for genotyping analysis. By analyzing STR profiles from reference samples, a reference STR table is generated, serving as a comparison tool for identifying repeat differences between the genotypes of test individuals. This comprehensive analysis of genetic variations provides valuable insights for differentiating between individuals based on their unique genetic profiles.

Keywords: Amplicon; Amplicon fragment analysis; DNA; FTA cards; Fragment; Genetic profiling; Genotyping; Leaf tissue; Lineage; PCR; Primers; Repeats; STR; Short tandem repeat (STR) analysis.

PubMed Disclaimer

Conflict of interest statement

Conflict of Interest. The authors have no conflicts of interest to declare that are relevant to the content of this chapter.

References

    1. Qu W, Wang F, Sun D, Liu Y, Jin X, Gong Z, Liu J, Zhang N, Wen D, Wang C, Jia H, Xu R, Tang X, Chen S, Fu X, Li X, Rong H, Zhang T, Jin C, Chen L, Li J, Liu Y, Cai J, Jiang B, Zha L (2024) Internal validation of the GA118-24B Genetic Analyzer, a stable capillary electrophoresis system for forensic DNA identification. Int J Legal Med 138(2):361–373. https://doi.org/10.1007/s00414-023-03106-x - DOI - PubMed
    1. Vasek J, Cilova D, Melounova M, Svoboda P, Zdenkova K, Cermakova E, Ovesna J (2021) OpiumPlex is a novel microsatellite system for profiling opium poppy (Papaver somniferum L.). Sci Rep 11(1):12799. https://doi.org/10.1038/s41598-021-91962-1 - DOI - PubMed - PMC
    1. Cateau E, Leclerc A, Cartier N, Valsecchi I, Bailly E, Le Senechal R, Becerra M, Le Gallou B, Lavergne RA, Chesnay A, Robin JP, Cray C, Goddard N, Thorel M, Guillot J, Mulot B, Desoubeaux G (2022) Aspergillosis in a colony of Humboldt penguins (Spheniscus humboldti) under managed care: a clinical and environmental investigation in a French zoological park. Med Mycol 60(7):myac046. https://doi.org/10.1093/mmy/myac046 - DOI - PubMed
    1. Testolin R, Messina R, Cipriani G, De Mori G (2023) SSR-based DNA fingerprinting of fruit crops. Crop Sci 63(2):390–459. https://doi.org/10.1002/csc2.20896 - DOI
    1. Driskill M, Pardee K, Hummer KE, Zurn JD, Amundsen K, Wiles A, Wiedow C, Patzak J, Henning JA, Bassil NV (2022) Two fingerprinting sets for Humulus lupulus based on KASP and microsatellite markers. PLoS One 17(4):e0257746. https://doi.org/10.1371/journal.pone.0257746 - DOI - PubMed - PMC

LinkOut - more resources