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. 2025 Jun 26;3(2):e100177.
doi: 10.1136/egastro-2024-100177. eCollection 2025.

Contribution of colibactin-producing Escherichia coli to colonic carcinogenesis

Hideki Ishikawa  1 , Ryogo Aoki  2 , Michihiro Mutoh  3   4 , Shingo Ishiguro  5 , Takuji Tanaka  2 , Noriyuki Miyoshi  6 , Shingo Miyamoto  3 , Takahiro Hamoya  3 , Naohisa Yoshida  7 , Keiji Wakabayashi  8 , Kenji Watanabe  6
Affiliations

Contribution of colibactin-producing Escherichia coli to colonic carcinogenesis

Hideki Ishikawa et al. eGastroenterology. .
No abstract available

Keywords: Colonic Polyps; Colorectal Neoplasms; Gastrointestinal Cancer; Gastrointestinal Microbiome; Intestinal Microbiota.

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Conflict of interest statement

None declared.

Figures

Figure 1
Figure 1. Representative histopathology of the polyps. A polyp lesion with colibactin-producing E. coli (pks+) is shown in panels A–C. (A) HE staining of the polyp lesion, which shows high-grade tubular adenoma. (B) High magnification of A. (C) A few bacteria with gram-positive and negative stains are seen in the crypts and cryptal lumen. A polyp lesion without colibactin-producing E. coli (pks−) is shown in D–F. (D) HE staining of the polyp lesion, which shows low-grade tubular adenoma. (E) High magnification of D. (F) Bacteria with gram-positive and negative stains are observed in the crypts and cryptal lumen. Figure permission is sought for the image.
Figure 2
Figure 2. Representative immunohistopathology of the polyps. Polyp lesions with colibactin-producing E. coli (pks+, A–D) and without colibactin-producing E. coli (pks−, E–H) are shown. (A) Lymphocytes showing CD3-positive reaction infiltrating the mucosal lamina propria. (E) CD5-positive lymphocytes infiltrating the mucosal lamina propria. (B and F) γ-H2AX immunostaining. (I) The positive rate (%) of nuclei to the total number of adenoma cell nuclei is shown. (C and G) IL-6 immunostaining. (J) The immunoreactivity score (IRS) value was evaluated by IRS = (staining percentage score) × (staining intensity score) and shown. (D and H) IL-1ß immunostaining. (K) IRS values are shown. Bars, 50 µm. Figure permission is sought for the image.
See this image and copyright information in PMC

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