Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1985 Oct 11;13(19):7119-28.
doi: 10.1093/nar/13.19.7119.

Synthesis and hybridization of dodecadeoxyribonucleotides containing a fluorescent pyridopyrimidine deoxynucleoside

H Inoue, A Imura, E Ohtsuka
Free PMC article

Synthesis and hybridization of dodecadeoxyribonucleotides containing a fluorescent pyridopyrimidine deoxynucleoside

H Inoue et al. Nucleic Acids Res. .
Free PMC article

Abstract

Partially self-complementary dodecadeoxyribonucleotides containing a fluorescent nucleoside, 3-beta-D-2'-deoxyribofuranosyl-2, 7-dioxopyrido[2, 3-d]pyrimidine (pyridopyrimidine deoxynucleoside, dF) were synthesized by the phosphotriester solidphase method. A dodecanucleotide d(GGGAAFGTTCCC) pairing the analog and guanine at the centre of the chain showed a higher melting temperature than the corresponding G-C paired duplex. A similar comparison between A-T and A-F suggested that weaker hydrogen bonds exist when adenine and pyridopyrimidine residues are paired.

PubMed Disclaimer

References

    1. Nucleic Acids Res. 1974 Mar;1(3):331-53 - PubMed
    1. Proc Natl Acad Sci U S A. 1973 Apr;70(4):1209-13 - PubMed
    1. Biochemistry. 1978 Nov 14;17(23):4894-9 - PubMed
    1. J Biol Chem. 1985 Mar 10;260(5):2605-8 - PubMed
    1. Proc Natl Acad Sci U S A. 1984 Oct;81(19):5956-60 - PubMed

MeSH terms

Substances