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. 2025:2937:325-360.
doi: 10.1007/978-1-0716-4591-8_19.

Competitive Epitope Binning Using HT-SPR

Daniel Bedinger  1 , Judicael Parisot  2 , Noah T Ditto  2
Affiliations

Competitive Epitope Binning Using HT-SPR

Daniel Bedinger et al. Methods Mol Biol. 2025.

Abstract

Competitive epitope binning using high-throughput surface plasmon resonance (HT-SPR) is a method for understanding competitive epitope profiles of up to hundreds of monoclonal antibodies. Detailed here is an approach along with considerations for the design and analysis of a classical competitive epitope binning assay using the Carterra LSA. Monoclonal antibodies are arrayed on a biosensor chip and interrogated serially for their ability to form a trimolecular complex with antigen and solution phase monoclonal antibodies. Software tools allow the clustering of monoclonal antibodies sharing common epitopes, and the real-time nature of the data allows for study of binding profiles for each monoclonal antibody.

Keywords: Biosensors; Classical sandwich binning; Competitive epitope binning; HT-SPR; Monoclonal antibodies; Premix binning; mAbs.

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