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. 2025 Jul 1;15(1):21073.
doi: 10.1038/s41598-025-07411-w.

Predictive value of AMH in late reproductive age: a retrospective cohort study

Nejc Kozar  1   2 , Vida Gavrić Lovrec  3   4 , Borut Kovačič  3 , Milan Reljič  3   4
Affiliations

Predictive value of AMH in late reproductive age: a retrospective cohort study

Nejc Kozar et al. Sci Rep. .

Abstract

This study evaluates the predictive value of Anti-Müllerian Hormone (AMH) levels on outcomes in medically assisted reproduction (MAR), with a focus on women of late reproductive age. AMH, produced by granulosa cells in ovarian follicles, serves as an important indicator of ovarian reserve. A retrospective cohort analysis was conducted, encompassing 4891 MAR cycles, to assess the age-specific predictive value of AMH for clinical pregnancy. The results indicate that AMH is significantly correlated with clinical pregnancy outcomes (p < 0.01) and demonstrates potential predictive value in women over 35 years of age, with its predictive capacity increasing notably with age (AUC = 0.62-0.69). However, in younger women, the correlation between AMH levels and MAR outcomes is weaker (AUC = 0.48-0.53). These findings highlight the importance of integrating AMH measurements with age when assessing fertility potential and tailoring MAR treatments, particularly for women approaching the end of their reproductive lifespan.

Keywords: AMH; Clinical pregnancy; IVF; Late reproductive age; MAR.

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Conflict of interest statement

Declarations. Competing interests: The authors declare no competing interests. Ethics approval: Use of the database was approved by the Medical Ethics Committee of UKC Maribor under approval number UKC-MB-KME-48/24.

Figures

Fig. 1
Fig. 1
No. of collected oocytes stratified by age.
Fig. 2
Fig. 2
Relationship between AMH concentrations and age.
Fig. 3
Fig. 3
Significance of correlation between clinical pregnancy and AMH levels, stratified by age.
Fig. 4
Fig. 4
ROC comparison.
See this image and copyright information in PMC

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