Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2025 Jul 1;25(1):971.
doi: 10.1186/s12903-025-06343-1.

Color stability of clear aligners exposed to various beverages: an in vitro study

Chanakarn Kobkiatkawin  1 , Woraphong Panyayong  1   2 , Suphachai Suphangul  1 , Sasipa Thiradilok  3
Affiliations

Color stability of clear aligners exposed to various beverages: an in vitro study

Chanakarn Kobkiatkawin et al. BMC Oral Health. .

Abstract

Background: Staining clear aligners from beverages commonly occurs and can affect a patient's self-esteem and confidence in social interactions. The aim of the present study was to evaluate the color stability of clear aligners when exposed to several types of beverages.

Methods: A total of 704 (seven hundred four, 176 per brand) clear aligner samples from four brands (Beforedent®, Käse Aligner®, Invisalign® and ClearCorrect®) were immersed in three types of beverages (tea, coffee and cola) and a control solution (artificial saliva) for 6 and 12 hours. The color before immersion (T0), after 6 hours (T1) and 12 hours after immersion (T2) were measured in the CIE L*-a -b system, the color difference (ΔE*) and the National Bureau of Standard Unit (NBS) were calculated. Fourier transform infrared spectroscopy (FTIR) and 3D laser scanning microscopy were performed to identify the polymer composition and surface properties. The data were analyzed via two-way ANOVA and multiple comparisons using Bonferroni corrections, with P < 0.05 indicating statistical significance.

Results: After 6 and 12 hours, Invisalign® in coffee and tea presented significantly greater color differences than ClearCorrect®, Beforedent®, and Käse Aligner® did. No significant color changes were observed in the cola or artificial saliva (control) for any of the brands. According to the FTIR analysis, Invisalign® is made from polyurethane, ClearCorrect® from polyester, and both Beforedent® and Käse Aligner® from polyethylene terephthalate. The highest degree of roughness and porosity was shown on the surface of Invisalign®. The spectral features of samples immersed in colored beverages almost overlapped with as receive samples before immersion.

Conclusions: Invisalign® made from polyurethane resulted in the most color changes, followed by ClearCorrect® Beforedent® and Käse Aligner® had the least and equivalent color changes. Coffee caused the most discoloration on clear aligners, followed by tea, cola, and artificial saliva (control). After 6 and 12 hours of immersion, all the groups presented good color stability, except for Invisalign® immersed in coffee for 6-12 hours, and ClearCorrect® immersed in coffee for 12 hours. The removal of clear aligner before beverage consumption is recommended.

Keywords: Clear aligners; Color difference; Fourier transform infrared; Orthodontic appliances; Spectroscopy; Surface properties.

PubMed Disclaimer

Conflict of interest statement

Declarations. Ethics approval and consent to participate: Approval by the Faculty of Dentistry/Faculty of Pharmacy, Mahidol University, Institution Review Board (Reference number: COE.No.MU-DTPY-IRB 2023/033.2607). Consent for publication: Not applicable. Competing interests: The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
Graph showing color differences (ΔE*) after 6 h immersion in all beverages
Fig. 2
Fig. 2
Graph showing color differences (ΔE*) after 12 h immersion in all beverages
Fig. 3
Fig. 3
Graph showing the NBS unit after 6 h immersion in all beverages
Fig. 4
Fig. 4
Graph showing the NBS unit after 12 h immersion in all beverages
Fig. 5
Fig. 5
The figures showed the FTIR spectra of the clear aligner: (A) Invisalign®, (B) Beforedent®, (C) Käse Aligner®, (D) ClearCorrect®
Fig. 6
Fig. 6
The figures showed the FTIR spectra of as-received Invisalign® compared with samples immersed in all beverages for 12 h
Fig. 7
Fig. 7
The figures showed the FTIR spectra of as-received ClearCorrect ®compared with samples immersed in all beverages for 12 h
Fig. 8
Fig. 8
The figures showed surface characteristics of Beforedent® before immersion in beverages: (A-C) Outer surface; (D-F) Inner surface
Fig. 9
Fig. 9
The figures showed surface characteristics of Käse Aligner® before immersion in beverages: (A-C) Outer surface; (D-F) Inner surface
Fig. 10
Fig. 10
The figures showed surface characteristics of Invisalign® before immersion in beverages: (A-C) Outer surface; (D-F) Inner surface
Fig. 11
Fig. 11
The figures showed surface characteristics of ClearCorrect® before immersion in beverages: (A-C) Outer surface; (D-F) Inner surface:
Fig. 12
Fig. 12
The figures showed surface characteristics of Beforedent ® after 12 h of immersion in beverages: Outer surface; (A) Coffee, (B) Tea, (C) Cola, (D) Artificial saliva Inner surface; (E) Coffee, (F) Tea, (G) Cola, (H) Artificial saliva
Fig. 13
Fig. 13
The figures showed surface characteristics of Käse Aligner® after 12 h of immersion beverages: Outer surface; (A) Coffee, (B) Tea, (C) Cola, (D) Artificial saliva Inner surface; (E) Coffee, (F) Tea, (G) Cola, (H) Artificial saliva
Fig. 14
Fig. 14
The figures showed surface characteristics of Invisalign® after 12 h of immersion in beverages: Outer surface; (A) Coffee, (B) Tea, (C) Cola, (D) Artificial saliva Inner surface; (E) Coffee, (F) Tea, (G) Cola, (H) Artificial saliva
Fig. 15
Fig. 15
The figures showed surface characteristics of ClearCorrect® after 12 h of immersion in beverages: Outer surface; (A) Coffee, (B) Tea, (C) Cola, (D) Artificial saliva Inner surface; (E) Coffee, (F) Tea, (G) Cola, (H) Artificial saliva
See this image and copyright information in PMC

Similar articles

See all similar articles

References

    1. Gold BP, Siva S, Duraisamy S, Idaayath A, Kannan R. Properties of orthodontic clear aligner Materials - A review. J Evol Med Dent Sci. 2021;10:3288–94.
    1. Shetty S, Shaikh N. Clear aligner therepy– A review. J Dent Spec. 2021;9:46–52.
    1. Šimunović L, Čekalović Agović S, Marić AJ, Bačić I, Klarić E, Uribe F, et al. Color and chemical stability of 3D-Printed and thermoformed Polyurethane-Based aligners. Polymers. 2024;16(8):1067. - PMC - PubMed
    1. Liu C-L, Sun W-T, Liao W, Lu W-X, Li Q-W, Jeong Y, et al. Colour stabilities of three types of orthodontic clear aligners exposed to staining agents. Int J Oral Sci. 2016;8(4):246–53. - PMC - PubMed
    1. Lagravère MO, Flores-Mir C. The treatment effects of invisalign orthodontic aligners: a systematic review. J Am Dent Assoc. 2005;136(12):1724–9. - PubMed

LinkOut - more resources