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. 2025 Jun 24:2025:2602317.
doi: 10.1155/ijm/2602317. eCollection 2025.

Comparative Evaluation of a Disinfectant Formulation Comprising Hydrogen Peroxide, Peracetic Acid, and Acetic Acid Against Aspergillus niger, Escherichia coli, and Staphylococcus aureus on Various Surfaces in Comparison to Other Disinfectants

Md Raisul Islam Rabby  1 , Md Sabbir Hossain  1 , Nafisa Nusrat Chowdhury  1 , Fatema Akter  1 , Mamudul Hasan Razu  1 , Zabed Bin Ahmed  1 , Mala Khan  1
Affiliations

Comparative Evaluation of a Disinfectant Formulation Comprising Hydrogen Peroxide, Peracetic Acid, and Acetic Acid Against Aspergillus niger, Escherichia coli, and Staphylococcus aureus on Various Surfaces in Comparison to Other Disinfectants

Md Raisul Islam Rabby et al. Int J Microbiol. .

Abstract

This study investigates the effectiveness of a disinfectant formulation comprising acetic acid, hydrogen peroxide, and peracetic acid (AAHPA) against Aspergillus niger, Escherichia coli, and Staphylococcus aureus on petri plates, smooth surfaces, and rough surfaces. Comparative analysis with conventional disinfectants such as 70% isopropyl alcohol, 1.5% chlorhexidine gluconate, 10% sodium hypochlorite, and 0.2% hydrogen peroxide reveal AAHPA's superior performance, achieving significantly higher log reduction (p < 0.05) and percentage reduction (p < 0.01) against A. niger in 5 min on petri plates. On smooth and rough surfaces, AAHPA exhibits exceptional efficacy, demonstrating the highest log and percentage reduction against A. niger in 0.5, 1, and 5 min, surpassing other disinfectants. AAHPA shows the highest bacterial decline against E. coli and S. aureus, followed by 0.2% hydrogen peroxide. In some cases, 0.2% hydrogen peroxide was slightly more effective than AAHPA against E. coli and S. aureus in 5 min. Time-dependent analysis of log reduction trends emphasizes AAHPA's rapid and consistent effectiveness, particularly in 0.5 min, positioning it as a promising disinfectant formulation with broad-spectrum efficacy across diverse surfaces.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
The log reduction of tested microbes and spores by five disinfectants on petri plates at different time intervals.
Figure 2
Figure 2
The log reduction of tested microbes and spores by five disinfectants on smooth surfaces at different time intervals.
Figure 3
Figure 3
The log reduction of tested microbes and spores by five disinfectants on rough surfaces at different time intervals.
Figure 4
Figure 4
Effectiveness of disinfectants in 0.5 min.
Figure 5
Figure 5
Surface efficacy of disinfectants (combined the log reductions of three contact times and the three organisms to measure the mean log reduction of each disinfectant on different surfaces).
See this image and copyright information in PMC

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