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. 2025 Aug 14;14(8):e0036325.
doi: 10.1128/mra.00363-25. Epub 2025 Jul 2.

Genomic characterization of Citrobacter freundii clinical isolates from Tanzania

Gerald Mboowa  1   2 , Benson R Kidenya  3 , Ivan Sserwadda  1   2 , Stephen Kanyerezi  1   2 , Inyasi Lawrence Akaro  4 , Baraka Mkinze  4 , Jeremiah Seni  5
Affiliations

Genomic characterization of Citrobacter freundii clinical isolates from Tanzania

Gerald Mboowa et al. Microbiol Resour Announc. .

Abstract

We report draft genomes of three Citrobacter freundii isolates from Tanzania. Genomes averaged 6.9 Mb with 51.49% GC content and 114× depth. ST167 carried multiple extended-spectrum β-lactamase, fluoroquinolone, and aminoglycoside resistance genes alongside virulence factors, highlighting the need for genomic surveillance of emerging multidrug-resistant C. freundii lineages.

Keywords: Citrobacter freundii; antimicrobial resistance (AMR); genomic surveillance; virulence factors; whole-genome sequencing.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Fig 1
Fig 1
(A–C) Genome circularization of draft C. freundii A56429, C. freundii A55880, and C. freundii A56400. The concentric rings display annotated genomic features, with the outermost ring showing coding sequences (CDS) and genes, including hypothetical proteins, annotated by Prokka on both strands. Functional elements are color-coded: CDS (purple), tRNA (orange), rRNA (cyan), AMR genes (green), and stress response genes (blue). Visualization was performed using Proksee (https://proksee.ca/). Genomic coordinates are marked in megabase pairs (Mbp) around the circle.
See this image and copyright information in PMC

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