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Review
. 2025 Jun 18:13:1621641.
doi: 10.3389/fbioe.2025.1621641. eCollection 2025.

Application of decellularization methods for scaffold production: advantages, disadvantages, biosafety and modifications

O I Shevchuk  1   2 , V V Korcheva  1 , N S Moskalenko  1 , V M Kyryk  1   3 , K V Kot  2 , D S Krasnienkov  1
Affiliations
Review

Application of decellularization methods for scaffold production: advantages, disadvantages, biosafety and modifications

O I Shevchuk et al. Front Bioeng Biotechnol. .

Abstract

The development of efficient, biocompatible scaffolds is an actual challenge in tissue engineering. Scaffolds derived from animal sources offer promising structural and biochemical properties but require thorough decellularization to minimize immunogenicity and maintain extracellular matrix integrity. Effective decellularization requires a synergistic combination of methods to ensure complete removal of immunogenic cellular components while preserving critical extracellular matrix elements such as glycosaminoglycans, collagens, and growth factors. This review covers the application of some decellularization methods (physical, chemical) in scaffold production, highlighting their respective advantages, limitations, and biosafety considerations. Moreover, the importance of scaffold sterilization: both physical techniques like gamma irradiation and chemical agents-are mentioned for their efficacy and cytotoxic risks. Furthermore, scaffold modifications, particularly recellularization strategies, are discussed as key enhancements to improve biocompatibility and functional integration. Overall, the selection and optimization of decellularization protocols are crucial for the safe and effective clinical implementation of bioengineered scaffolds.

Keywords: biocompatibility; decellularization; non-animal scaffold production; scaffold; scaffold modification.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

FIGURE 1
FIGURE 1
Scheme of tissues freeze-thaw cycling during decellularization process.
FIGURE 2
FIGURE 2
Cell lysis during in vivo electroporation.
FIGURE 3
FIGURE 3
Disrupting cellular membrane by detergents.
See this image and copyright information in PMC

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