Minocycline reduces proinflammatory and oxidative stress markers in the spinal cord and morphology changes in sciatic nerve of Type 2 diabetic neuropathy rat model
- PMID: 40607152
- PMCID: PMC12209144
- DOI: 10.1007/s13340-025-00811-3
Minocycline reduces proinflammatory and oxidative stress markers in the spinal cord and morphology changes in sciatic nerve of Type 2 diabetic neuropathy rat model
Abstract
Aim: This study investigated the effects of minocycline on proinflammatory cytokines, oxidative stress marker levels in the spinal cord and sciatic nerve morphology in Type 2 diabetic (T2DM) neuropathy rats.
Methods: Male Sprague Dawley rats were randomly assigned to six groups (n = 14 per groups): Control (C), T2DM control (STZ), T2DM treated with minocycline 40 mg/kg (STZ + M40) and 80 mg/kg (STZ + M80), T2DM treated with gabapentin (STZ + G10) and non-painful T2DM neuropathy (NPDN). T2DM was induced in obese rats using a combination of high fat diet (HFD) and low-dose streptozotocin (STZ) (40 mg/kg) injection. Then, the neuropathic pain behaviour, body weight and blood biochemical analysis were performed. Rats were sacrificed and the spinal cord and sciatic nerve were collected for ELISA and histology examination.
Results: T2DM rat groups were significantly increased body weight after 6 weeks but significantly reduced from 8 until 9 weeks compared to control group (p < 0.05). The fasting blood glucose (FBG) level in all T2DM groups were significantly higher on day 3, day 14, and day 22 compared to control group (p < 0.05) consistent with HbA1c levels. T2DM groups also significantly increased MDA, TNF-α, IL-1β and C-Reactive Protein (CRP) but decreased SOD and Catalase levels in the spinal cord compared to control group (p < 0.05). T2DM groups also showed significant abnormal morphology changes in the sciatic nerve compared to control group (p < 0.05). Minocycline dependent on doses and gabapentin in T2DM rat significantly alleviated all these effects.
Conclusion: These findings suggest the neuroprotective effects of minocycline on T2DM neuropathy.
Keywords: Biochemical analysis; Minocycline; Oxidative stress marker; Pro-inflammatory cytokines; Sciatic nerve histology; Type 2 diabetic neuropathy.
© The Japan Diabetes Society 2025. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
Conflict of interest statement
Conflict of interestThe author(s) declared no potential conflicts of interest concerning the research, authorship, and/or publication of this article.
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