Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2025 Jul 3:10.1111/cea.70108.
doi: 10.1111/cea.70108. Online ahead of print.

Efficacy of the 4 Subclasses of IgG for Interacting With CD32b/a on Human Basophils

Donald MacGlashan Jr  1 , Benjamin W Spiller  2 , Scott A Smith  3
Affiliations

Efficacy of the 4 Subclasses of IgG for Interacting With CD32b/a on Human Basophils

Donald MacGlashan Jr et al. Clin Exp Allergy. .

Abstract

- IgG3 and IgG4 most effectively inhibit basophil activation through CD32b engagement.

- IgG2 shows minimal CD32b-dependent inhibition at physiologically relevant concentrations.

PubMed Disclaimer

Conflict of interest statement

Conflicts of Interest statement: SAS receives royalties from InBio Inc for intellectual property and is a paid consultant for IgGenix Inc. SAS is an inventor on a patent entitled “Generation of human allergen- and helminth-specific IgE monoclonal antibodies for diagnostic and therapeutic use” (U.S. patent no. US10908168-B2) and on a pending patent entitled “Generation of Human Peanut Allergen-Specific IgE Monoclonal Antibodies for Diagnostic and Therapeutic Use” (63/159,764). Royalties have been paid for both patents. BWS is a co-founder and owner at Turkey Creek Biotechnology (TCB). TCB was not involved in this work.

References

    1. Cady CT, Powell MS, Harbeck RJ, Giclas PC, Murphy JR, Katial RK, et al. IgG antibodies produced during subcutaneous allergen immunotherapy mediate inhibition of basophil activation via a mechanism involving both FcgammaRIIA and FcgammaRIIB. Immunol Lett. 2010;130(1–2):57–65. - PMC - PubMed
    1. MacGlashan D Jr., Moore G, Muchhal U. Regulation of IgE-mediated signalling in human basophils by CD32b and its role in Syk down-regulation: basic mechanisms in allergic disease. Clin Exp Allergy. 2014;44(5):713–23. - PubMed
    1. MacGlashan D Jr., Hamilton RG. Parameters determining the efficacy of CD32 to inhibit activation of FcepsilonRI in human basophils. J Allergy Clin Immunol. 2016;137(4):1256–8 e1–11. - PMC - PubMed
    1. Smith K, Garman L, Wrammert J, Zheng NY, Capra JD, Ahmed R, et al. Rapid generation of fully human monoclonal antibodies specific to a vaccinating antigen. Nature protocols. 2009;4(3):372–84. - PMC - PubMed
    1. Croote D, Darmanis S, Nadeau KC, Quake SR. High-affinity allergen-specific human antibodies cloned from single IgE B cell transcriptomes. Science. 2018;362(6420):1306–9. - PubMed

LinkOut - more resources