Growth factor independent 1B is a metabolic regulator that exerts pleiotropic effects on platelet function

Marie-Christine Morel-Kopp¹, Marie Levade², Lucinda Beutler², Qiang Chen¹, Jad Othman³, Pascal Steffen⁴, Matthew J McKay⁴, Mark P Molloy⁴, Maria V Selvadurai⁵, Helena P H Liang⁶, Shane Whittaker⁶, Dianne E van der Wal⁶, Sara Gabrielli², Justin R Hamilton⁵, Vivien M Chen⁶, Ashley P Ng⁷, Christopher M Ward¹, William S Stevenson⁸

Affiliations

¹ Northern Blood Research Centre, Kolling Institute, The University of Sydney, Sydney, New South Wales, Australia; Department of Haematology and Transfusion Medicine, Royal North Shore Hospital, Sydney, New South Wales, Australia.
² Northern Blood Research Centre, Kolling Institute, The University of Sydney, Sydney, New South Wales, Australia.
³ Department of Haematology and Transfusion Medicine, Royal North Shore Hospital, Sydney, New South Wales, Australia.
⁴ Bowel Cancer and Biomarker Laboratory, Kolling Institute, School of Medical Sciences, The University of Sydney, Sydney, New South Wales, Australia.
⁵ Australian Centre for Blood Diseases, Monash University, Melbourne, Victoria, Australia.
⁶ Platelet and Thrombosis Research Group, Australian and New Zealand Army Corps Research Institute, Sydney Local Health District, University of Sydney, Sydney, New South Wales, Australia; Department of Haematology, Concord General and Repatriation Hospital, Concord, Sydney, New South Wales, Australia.
⁷ Cancer and Haematology Division, The Walter and Eliza Hall Institute, The University of Melbourne, Melbourne, Victoria, Australia.
⁸ Northern Blood Research Centre, Kolling Institute, The University of Sydney, Sydney, New South Wales, Australia; Department of Haematology and Transfusion Medicine, Royal North Shore Hospital, Sydney, New South Wales, Australia. Electronic address: william.stevenson@sydney.edu.au.

PMID: 40609904
DOI: 10.1016/j.jtha.2025.06.021

Growth factor independent 1B is a metabolic regulator that exerts pleiotropic effects on platelet function

Marie-Christine Morel-Kopp et al. J Thromb Haemost. 2025 Oct.

. 2025 Oct;23(10):3349-3361.

doi: 10.1016/j.jtha.2025.06.021. Epub 2025 Jul 1.

Authors

Affiliations

¹ Northern Blood Research Centre, Kolling Institute, The University of Sydney, Sydney, New South Wales, Australia; Department of Haematology and Transfusion Medicine, Royal North Shore Hospital, Sydney, New South Wales, Australia.
² Northern Blood Research Centre, Kolling Institute, The University of Sydney, Sydney, New South Wales, Australia.
³ Department of Haematology and Transfusion Medicine, Royal North Shore Hospital, Sydney, New South Wales, Australia.
⁴ Bowel Cancer and Biomarker Laboratory, Kolling Institute, School of Medical Sciences, The University of Sydney, Sydney, New South Wales, Australia.
⁵ Australian Centre for Blood Diseases, Monash University, Melbourne, Victoria, Australia.
⁶ Platelet and Thrombosis Research Group, Australian and New Zealand Army Corps Research Institute, Sydney Local Health District, University of Sydney, Sydney, New South Wales, Australia; Department of Haematology, Concord General and Repatriation Hospital, Concord, Sydney, New South Wales, Australia.
⁷ Cancer and Haematology Division, The Walter and Eliza Hall Institute, The University of Melbourne, Melbourne, Victoria, Australia.
⁸ Northern Blood Research Centre, Kolling Institute, The University of Sydney, Sydney, New South Wales, Australia; Department of Haematology and Transfusion Medicine, Royal North Shore Hospital, Sydney, New South Wales, Australia. Electronic address: william.stevenson@sydney.edu.au.

PMID: 40609904
DOI: 10.1016/j.jtha.2025.06.021

Abstract

Background: Human variants in the growth factor independent 1B (GFI1B) transcription factor are associated with thrombocytopenia and a variable bleeding phenotype.

Objectives: In this study, we examined the bleeding phenotype associated with the human GFI1B^H294fs variant and compared it with a mouse model of abnormal GFI1B function.

Methods: The transcriptomic and proteomic profiles of human GFI1B^H294fs platelets were analyzed and compared with those of murine megakaryocytes harboring conditional deletion of Gfi1b. Observed changes were subsequently validated with imaging and western blotting and in a cell model of endocytosis.

Results: All individuals with the GFI1B^H294fs variant displayed markedly impaired or absent platelet aggregation responses to collagen stimulation as assessed by light transmission aggregometry. Mice with conditional deletion of Gfi1b in the megakaryocyte lineage had severe thrombocytopenia with increased numbers of abnormal megakaryocytes and progenitors. Proteomic pathway analysis of human platelets and mouse megakaryocytes revealed defects in platelet aggregation and spreading, dysregulated glycoprotein VI and integrin signaling, and reduced endocytosis. Megakaryocytes deficient in Gfi1b displayed structural abnormalities and endocytosis failure with decreased fibrinogen uptake from surrounding media consistent with the fibrinogen deficiency observed in human GFI1B^H294fs platelets. Unexpectedly, oxidative phosphorylation was upregulated in both platelets and megakaryocytes, and this was associated with mitochondrial hyperplasia on cell imaging. Pyruvate kinase M2 emerged as a potential mediator of these metabolic changes.

Conclusion: Dysregulated GFI1B function affects multiple biological pathways linked to abnormal bleeding. Reduced GFI1B activity also induces metabolic reprogramming in platelets and megakaryocytes, leading to enhanced oxidative phosphorylation.

Keywords: GFI1B; blood platelets; mitochondria; oxidative phosphorylation; thrombocytopenia.

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Conflict of interest statement

Declaration of competing interests There are no competing interests to disclose.

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Growth factor independent 1B is a metabolic regulator that exerts pleiotropic effects on platelet function

Affiliations

Growth factor independent 1B is a metabolic regulator that exerts pleiotropic effects on platelet function

Authors

Affiliations

Abstract

Conflict of interest statement

MeSH terms

Substances

LinkOut - more resources

Full Text Sources

Medical