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. 1985 Aug 1;148(2):269-76.
doi: 10.1016/0003-2697(85)90228-3.

Determination of nucleotide pools in plant tissue by high-performance liquid chromatography

R Meyer, K G Wagner

Determination of nucleotide pools in plant tissue by high-performance liquid chromatography

R Meyer et al. Anal Biochem. .

Abstract

A procedure for the determination of nucleotide pools in plant tissue by HPLC is described. Sample preparation includes the extraction with 0.4 M HClO4, a purification step, which proved to be essential, on a disposable prepacked phenyl-bonded column, neutralization by KOH, and concentration by freeze-drying. The determination of a broad spectrum of ribonucleotides including the ribonucleosides was performed by combining anion-exchange and reversed-phase HPLC. Data are presented for suspension-cultured cells of Nicotiana tabacum and Datura innoxia and for the leaf and root of tobacco.

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