Potential of miR-192-5p as a diagnostic marker for children with severe pneumonia and respiratory failure and its predictive value for prognosis

Abstract

Introduction: Severe pneumonia in children is a rapidly progressing respiratory system disease. If not promptly controlled, it can lead to respiratory failure, posing a serious threat to the child's life. This study investigated the diagnostic and prognostic potential of miR-192-5p in children with severe pneumonia and respiratory failure.

Material and methods: A total of 62 children with severe pneumonia, 40 children with severe pneumonia and respiratory failure, and 62 healthy children were enrolled. The level of miR-192-5p was quantified by RT-qPCR assays. The diagnostic potential of miR-192-5p for severe pneumonia and respiratory failure was assessed through ROC curve and binary logistic analyses. The association between abnormal miR-192-5p level and prognosis of children with severe pneumonia with respiratory failure was evaluated by Kaplan-Meier and multivariate Cox analysis.

Results: miR-192-5p expression was decreased in the serum of severe pneumonia and expiratory failure children. miR-192-5p has a good potential to predict the occurrence of severe pneumonia, which is a risk predictor of severe pneumonia in children with respiratory failure. In addition, downregulation of miR-192-5p was strongly associated with poor prognosis of children with severe pneumonia and respiratory failure.

Conclusions: A low level of miR-192-5p has high predictive value and clinical development potential for the timely diagnosis and prediction of poor prognosis of children with severe pneumonia and respiratory failure.

Keywords: diagnosis; miR-192-5p; prognosis; respiratory failure; severe pneumonia.

Fig. 1

Expression and diagnostic analysis of miR-192-5p. A) miR-192-5p expression in the serum of children with severe pneumonia was lower than that of healthy children and higher than that of children with severe pneumonia and respiratory failure (***p < 0.001 vs. healthy; ###p < 0.001 vs. severe pneumonia). B, C) ROC analysis indicated the predictive ability with miR-192-5p in severe pneumonia (AUC = 0.939) and respiratory failure (AUC = 0.907)

Fig. 2

Relationship between miR-192-5p and prognosis in children with severe pneumonia and respiratory failure. A) miR-192-5p level was downregulated in the poor prognosis group of children with severe pneumonia and respiratory failure compared to the good prognosis group (***p < 0.001 vs. good prognosis). B) Low expression of miR-192-5p indicated poor prognosis in children with severe pneumonia and respiratory failure