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Case Reports
. 2025 Oct;44(10):2421-2426.
doi: 10.1007/s10096-025-05209-2. Epub 2025 Jul 7.

Molecular investigation of unexpected Helicobacter pylori bacteremia

Claudie Perreau  1 , Anne Gaelle Ranc  2 , Léo Gillet  1 , Tiphaine Gaillard  2 , Lucie Bénéjat  1 , Astrid Ducournau  1 , Johanna Aptel  1 , Quentin Jehanne  1 , Chantal Sobas  2 , Marine Jauvain  1   3 , Philippe Lehours  4   5   6
Affiliations
Case Reports

Molecular investigation of unexpected Helicobacter pylori bacteremia

Claudie Perreau et al. Eur J Clin Microbiol Infect Dis. 2025 Oct.

Abstract

This study describes the isolation of Helicobacter pylori from a blood sample of an 88-year-old patient with no history or clinical signs suggestive of this infection. We summarize the microbiological characterization of the case, and the subsequent investigation using advanced next-generation sequencing methods, including a target enrichment technique adapted for paraffin-embedded gastric biopsy analysis.

Keywords: Helicobacter pylori; Bacteremia; Next-generation sequencing.

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Conflict of interest statement

Declarations. Human ethics and consent to participate: All diagnostic methods were performed as part of routine clinical care. Remnants of specimens were preserved at the Centre de Ressources Biologiques-Bordeaux Biothèque Santé of Bordeaux University Hospital under contract number 17DMIP017_6 with no information regarding patient identity. All patient data were anonymously reported. Consent for publication: The patient provided written informed consent for the publication of this article. Competing interests: The authors declare no competing interests. Conflict of interest: The authors declare that they have no conflicts of interest.

Figures

Fig. 1
Fig. 1
Circular representation of sequencing results obtained through target enrichment followed by NGS on DNA extracted from the FFPE gastric biopsy The overall sequencing quality of all targeted regions is depicted using a circular representation. Fold coverage depth is color-coded as follows: green for positions with a depth of > 10-fold, yellow for positions with a depth between 5- and 10-fold, and red for positions with a depth of < 5-fold. Drops in depth are expected in regions where the sample’s genotype differs from the J99 reference strain genotype (i.e., vacA, cagA)
See this image and copyright information in PMC

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