De novo synthesis and glycosylation of the MOPC-46B mouse immunoglobulin light chain in cell-free extracts
- PMID: 406260
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De novo synthesis and glycosylation of the MOPC-46B mouse immunoglobulin light chain in cell-free extracts
J Biol Chem.
.
Free article
Abstract
The mRNA for the mouse MOPC-46B K chain, a glycorportein, was prepared translated in Ehrlich ascites cell-free extract. A polypeptide product similar to the MOPC-46B kappa chain was identified by its antigenic properties and by analysis of the products of tryptic digestion. In addition, the cell-free product was glycosylated after translation. It appeared that glycosylation of the cell-free product occurred on the same tryptic peptide glycosylated by intact cells. The characteristics and requirements of glycosylation are described. The presence of Mn2+ was a major requirement.
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