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. 2025 Jul 1:2025:8862271.
doi: 10.1155/tbed/8862271. eCollection 2025.

Prevalence of Methicillin-Resistant Staphylococcus aureus (MRSA) in Egyptian Water Buffaloes and Risk Factors for Subclinical Mastitis

Abdelfattah Selim  1 , Mohamed Marzok  2 , Hattan S Gattan  3   4 , Abdelrahman M Hereba  5
Affiliations

Prevalence of Methicillin-Resistant Staphylococcus aureus (MRSA) in Egyptian Water Buffaloes and Risk Factors for Subclinical Mastitis

Abdelfattah Selim et al. Transbound Emerg Dis. .

Abstract

Staphylococcus aureus (S. aureus) is one of the main causative agents of mastitis, which results in severe economic losses. In addition, methicillin-resistant S. aureus (MRSA) has been reported in dairy farms and in water buffaloes. The present study aimed to determine the prevalence of subclinical mastitis (SCM) in water buffaloes, associated risk factors for SCM, and prevalence of MRSA in positive milk samples for SCM. Milk samples (n = 385) from buffaloes were examined using the California mastitis test (CMT), and S. aureus was detected in positive milk samples using bacteriological and biochemical tests. In addition, MRSA was identified in positive S. aureus samples using PCR targeting the mecA gene. The results revealed that the prevalence of SCM among water buffaloes in the studied areas was 43.6%, and 61.9% (104/168) were identified as MRSA based on PCR targeting the mecA gene. In vitro antibiotic susceptibility testing found cefoxitin to be resistant and linezolid to be sensitive against MRSA isolates. In addition, the statistical analysis revealed that there was no significant association between the prevalence of SCM and locality or duration of lactation. The prevalence of SCM was strongly associated with age, parity, absence of teat dipping, hand cleaning of milker hands between milking, and in animals with a history of mastitis. Regular CMT can detect early SCM and improve udder sanitation and milking hygiene. In addition, continuous testing of antimicrobial drugs against MRSA isolates is necessary due to the importance of S. aureus in public health and the development of antibiotic resistance, such as methicillin.

Keywords: Egypt; PCR; Staphylococcus aureus; antibiotic-resistant; subclinical mastitis; water buffaloes.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
Map showed governorates under the study (map generated by QGis software).
Figure 2
Figure 2
Results of PCR products of mecA gene, lane M is a 100 bp DNA ladder, and lane 1 is a positive control. Lane 2–10 are positive samples for MRSA.
See this image and copyright information in PMC

References

    1. Radwan M. A. A. Cairo University; 2016. Characterization of Milk and Veal Production Chains of Buffalo Under Crop Livestock Production System in Egypt.
    1. Shahin A. S. A. H. Adoption of Innovations in Smallholder Buffalo Dairy Farms in the Menoufia Province in Egypt . Vol. 45. Köster; 2004.
    1. Selim A., Alshammari A., Gattan H. S., Alruhaili M. H., Rashed G. A., Shoulah S. Seroprevalence and Associated Risk Factors for Toxoplasma Gondii in Water Buffaloes (Bubalus bubalis) in Egypt. Comparative Immunology, Microbiology and Infectious Diseases . 2023;101 doi: 10.1016/j.cimid.2023.102058.102058 - DOI - PubMed
    1. Selim A., Marawan M. A., Ali A.-F., Manaa E., AbouelGhaut H. A. Seroprevalence of Bovine Leukemia Virus in Cattle, Buffalo, and Camel in Egypt. Tropical Animal Health and Production . 2020;52(3):1207–1210. doi: 10.1007/s11250-019-02105-8. - DOI - PubMed
    1. Benić M., Maćešić N., Cvetnić L., et al. Bovine Mastitis: A Persistent and Evolving Problem Requiring Novel Approaches for its Control-a Review. Veterinarski Arhiv . 2018;88(4):535–557. doi: 10.24099/vet.arhiv.0116. - DOI

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