doi: 10.1016/0005-2736(85)90168-3.
Size analysis of biological membrane vesicles by gel filtration, dynamic light scattering and electron microscopy
- PMID: 4063358
- DOI: 10.1016/0005-2736(85)90168-3
Item in Clipboard
Size analysis of biological membrane vesicles by gel filtration, dynamic light scattering and electron microscopy
Biochim Biophys Acta.
.
Abstract
Biological membrane vesicles are analysed in terms of size and size distribution using gel filtration on Sephacryl S-1000, electron microscopy and quasi-elastic light scattering. The agreement between the three methods is satisfactory particularly for homogeneous dispersions. Gel filtration on Sephacryl S-1000 is a quick and convenient method for the routine size analysis of membrane vesicles up to a diameter of about 250 nm.
Similar articles
-
Dynamic light scattering from polydisperse suspensions of thin ellipsoidal shells of revolution with submicron diameters.Biophys J. 1988 Apr;53(4):497-503. doi: 10.1016/S0006-3495(88)83129-1. Biophys J. 1988. PMID: 3382709 Free PMC article.
-
Cholesterol-phospholipid vesicles in human bile: an ultrastructural study.Biochim Biophys Acta. 1986 Oct 24;879(1):14-21. doi: 10.1016/0005-2760(86)90260-2. Biochim Biophys Acta. 1986. PMID: 3768382
-
Purification of microvillus membrane vesicles from pig small intestine by adsorption chromatography on Sepharose.Biochim Biophys Acta. 1983 Jan 19;727(2):412-5. doi: 10.1016/0005-2736(83)90426-1. Biochim Biophys Acta. 1983. PMID: 6838880 No abstract available.
-
Sizing of a vesicle drug formulation by quasi-elastic light scattering and comparison with electron microscopy and ultracentrifugation.J Pharm Sci. 1987 Jan;76(1):56-9. doi: 10.1002/jps.2600760116. J Pharm Sci. 1987. PMID: 3585725
-
Probes of membrane structure.Annu Rev Biochem. 1978;47:359-83. doi: 10.1146/annurev.bi.47.070178.002043. Annu Rev Biochem. 1978. PMID: 209727 Review. No abstract available.
Cited by
-
Osmotic water permeabilities of brush border and basolateral membrane vesicles from rat renal cortex and small intestine.J Membr Biol. 1986;92(2):183-93. doi: 10.1007/BF01870707. J Membr Biol. 1986. PMID: 3761362
-
Vesicle sizing: Number distributions by dynamic light scattering.Biophys J. 1991 Feb;59(2):357-62. doi: 10.1016/S0006-3495(91)82229-9. Biophys J. 1991. PMID: 19431789 Free PMC article.
-
Determination of the size distribution of liposomes by SEC fractionation, and PCS analysis and enzymatic assay of lipid content.AAPS PharmSciTech. 2002;3(2):E7. doi: 10.1208/pt030207. AAPS PharmSciTech. 2002. PMID: 12916944 Free PMC article.
-
Characterization of an integral protein of the brush border membrane mediating the transport of divalent metal ions.Biophys J. 2000 Aug;79(2):874-84. doi: 10.1016/S0006-3495(00)76343-0. Biophys J. 2000. PMID: 10920019 Free PMC article.
-
Synaptic vesicles studied by dynamic light scattering.Eur Phys J E Soft Matter. 2011 Jun;34(6):63. doi: 10.1140/epje/i2011-11063-2. Epub 2011 Jun 27. Eur Phys J E Soft Matter. 2011. PMID: 21706281
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources
Other Literature Sources
