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. 1985 Nov 21;821(1):169-73.
doi: 10.1016/0005-2736(85)90168-3.

Size analysis of biological membrane vesicles by gel filtration, dynamic light scattering and electron microscopy

G Perevucnik, P Schurtenberger, D D Lasic, H Hauser

Size analysis of biological membrane vesicles by gel filtration, dynamic light scattering and electron microscopy

G Perevucnik et al. Biochim Biophys Acta. .

Abstract

Biological membrane vesicles are analysed in terms of size and size distribution using gel filtration on Sephacryl S-1000, electron microscopy and quasi-elastic light scattering. The agreement between the three methods is satisfactory particularly for homogeneous dispersions. Gel filtration on Sephacryl S-1000 is a quick and convenient method for the routine size analysis of membrane vesicles up to a diameter of about 250 nm.

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