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. 2025 Sep:44:265-271.
doi: 10.1016/j.jgar.2025.07.002. Epub 2025 Jul 7.

Genomic characterization of carbapenem-resistant Pseudomonas aeruginosa from ICU admission screening in Hanoi, Vietnam, 2023

Lisa Göpel  1 , Truong Nhat My  2 , Kaan Kocer  3 , Thi Anh Mai Pham  1 , Le Thi Kieu Linh  4 , Bui Tien Sy  2 , Leo Huber  1 , Tran Thanh Tung  5 , Nguyen Trong The  2 , Le Huu Song  6 , Sébastien Boutin  7 , Thirumalaisamy P Velavan  8 , Dennis Nurjadi  9
Affiliations
Free article

Genomic characterization of carbapenem-resistant Pseudomonas aeruginosa from ICU admission screening in Hanoi, Vietnam, 2023

Lisa Göpel et al. J Glob Antimicrob Resist. 2025 Sep.
Free article

Abstract

Objective: Vietnam is among the countries most affected by antimicrobial resistance in the Asia-Pacific. While multidrug-resistant Enterobacterales have been extensively studied, genomic data on multidrug-resistant Pseudomonas aeruginosa in Vietnam remains scarce. To address this, we characterized 20 carbapenem-resistant P. aeruginosa (CRPA) isolates from rectal colonization of ICU patients.

Methods: Screening for CRPA was conducted using a selective chromogenic medium (mSuperCARBA). Species identification was achieved through MALDI-TOF mass spectrometry, while antimicrobial susceptibility testing was performed using the Vitek®2 system and broth microdilution. Whole-genome sequencing was performed using the Illumina NextSeq platform.

Results: Twenty CRPA isolates were collected from rectal swabs of 691 patients admitted to the ICUs of the 108 Military Central Hospital in Hanoi, Vietnam, between 1 July 2023 and 31 October 2023. The predominant multilocus sequence type was ST308, accounting for 50% (10/20) of the isolates. Notably, 70% (14/20) of the CRPA isolates harboured genes encoding metallo-β-lactamases (MBL), with blaNDM-1 being the most prevalent (86%, 12/14), followed by blaIMP-26 (14%, 2/14). Low susceptibility was observed for ceftazidime-avibactam (15%, 3/20) and ceftolozane-tazobactam (10%, 2/20), while cefiderocol resistance was observed in 50% (10/20) of isolates. Colistin demonstrated the most favourable susceptibility profile, with 90% (18/20) of isolates remaining susceptible.

Conclusions: A significant proportion of CRPA isolates in our study were MBL producers, with high levels of resistance to novel β-lactams and β-lactam/β-lactamase inhibitor combinations. These findings underscore the urgent need for effective infection prevention and control strategies to mitigate the further spread of MBL-producing CRPA.

Keywords: Admission screening; Carbapenem-resistant Pseudomonas aeruginosa; Genomic investigation; Metallo-β-lactamase; NDM; Vietnam.

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