Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2025 Apr 4;15(1):186-193.
doi: 10.34172/apb.43919. eCollection 2025 Apr.

Impact of Elateriospermum tapos Supplementation on Leptin and Hypothalamic Signaling in Female Offspring of High-Fat Diet-Induced Obese

Santhra Segaran Balan  1 , Hasnah Bahari  2 , Azrina Zainal Abidin  1 , Nurul Husna Shafie  3 , Maizaton Atmadini Abdullah  4 , Azmiza Syawani Jasni  5
Affiliations

Impact of Elateriospermum tapos Supplementation on Leptin and Hypothalamic Signaling in Female Offspring of High-Fat Diet-Induced Obese

Santhra Segaran Balan et al. Adv Pharm Bull. .

Abstract

Purpose: The central nervous system plays a crucial role in regulating food intake and energy expenditure to maintain energy homeostasis in the body. With rising obesity rates, alternative therapeutic strategies, including herbal-based interventions, are gaining attention. Elateriospermum tapos a plant that rich in flavonoids, has shown potential supporting weight reduction. This study aimed to evaluate the effects of E. tapos seed and shell supplementation on the hypothalamic feeding pathway in obese female rats and their offspring.

Methods: Thirty adult female Sprague-Dawley rats were used in this study. Obesity was induced in 24 rats via high-fat diet (HFD) for five weeks. Six rats were maintained on a normal diet as the control group (DCG). The obese rats were then divided into four groups: negative control (DNG, HFD only), positive control (DPG, HFD+orlistat 200 mg/kg), treatment 1 (DTX1, HFD+E. tapos seed 200 mg/kg), and treatment 2 (DTX2, HFD+E. tapos shell 200 mg/kg). Treatments were administered daily for six weeks before mating. On postnatal day 21 (PND21), blood and hypothalamus samples were collected from female rats and their female offspring. Plasma leptin levels were measured using ELISA, and expression of leptin receptor (Obr), proopiomelanocortin (POMC), and neuropeptide Y (NPY) in the hypothalamus was assessed by western blotting.

Results: DTX2 and offspring (OTX2) groups showed significantly (P<0.05) lower levels of leptin. Western blot results indicate Obr, POMC and NPY protein significantly (P<0.05) higher expression in DNG and ONG compared to the other groups.

Conclusion: In conclusion, the E. tapos shell significantly reduced maternal obesity in female offspring at PND21 compared to its seed.

Keywords: Elateriospermum tapos; Hypothalamus; Leptin; Maternal obesity; Obesity.

PubMed Disclaimer

Conflict of interest statement

No potential conflict of interest relevant to this article was reported.

Figures

Figure 1
Figure 1
Figure 2
Figure 2
Figure 3
Figure 3
Figure 4
Figure 4
Figure 5
Figure 5
Figure 6
Figure 6
See this image and copyright information in PMC

Similar articles

See all similar articles

References

    1. Chooi YC, Ding C, Magkos F. The epidemiology of obesity. Metabolism. 2019;92:6–10. doi: 10.1016/j.metabol.2018.09.005. - DOI - PubMed
    1. Bammann K, Peplies J, De Henauw S, Hunsberger M, Molnar D, Moreno LA, et al. Early life course risk factors for childhood obesity: the IDEFICS case-control study. PLoS One. 2014;9(2):e86914. doi: 10.1371/journal.pone.0086914. - DOI - PMC - PubMed
    1. Kulhanek D, Weigel R, Paulsen ME. Maternal high-fat-high-carbohydrate diet-induced obesity is associated with increased appetite in peripubertal male but not female C57Bl/6J mice. Nutrients. 2020;12(10):2919. doi: 10.3390/nu12102919. - DOI - PMC - PubMed
    1. Bharti A, Kulshrestha K. Childhood obesity: an overview. Int J Curr Microbiol Appl Sci. 2019;8(1):963–80. doi: 10.20546/ijcmas.2019.801.105. - DOI
    1. Engin A. The mechanism of leptin resistance in obesity and therapeutic perspective. Adv Exp Med Biol. 2024;1460:463–87. doi: 10.1007/978-3-031-63657-8_16. - DOI - PubMed

LinkOut - more resources