Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2025 Jun 26:16:1620907.
doi: 10.3389/fmicb.2025.1620907. eCollection 2025.

Characterization of bla NDM-5-carrying plasmids in two clinical Salmonella isolates from Jiaxing city, China

Ping Li #  1 , Yongjuan Yuan #  2 , Yong Yan  1 , Miaomiao Jia  1 , Lei Gao  1 , Xuejuan Liu  1 , Yangming Sun  1 , Guoying Zhu  1 , Zhongwen Chen  1
Affiliations

Characterization of bla NDM-5-carrying plasmids in two clinical Salmonella isolates from Jiaxing city, China

Ping Li et al. Front Microbiol. .

Abstract

Introduction: Salmonella is an important cause of foodborne diarrheal diseases worldwide. The emergence of bla NDM-positive carbapenem-resistant Salmonella enterica isolates in recent years poses a huge public health challenge.

Methods: In this study, two clinical S. enterica isolates carrying bla NDM-5: a serotype 4,[5],12:i:- strain (2023JX045) and a serovar Stanley strain (2024-406) were analyzed using antimicrobial susceptibility testing and whole genome sequencing.

Results: Both isolates were multidrug resistant, with insusceptibility to ampicillin, ampicillin/sulbactam, amoxicillin/clavulanic acid, cefuroxime, ceftiofur, cefazolin, cefoxitin, cefotaxime, ceftazidime, cefepime, ceftazidime/avibactam, meropenem, imipenem, ertapenem, tetracycline, gentamicin, trimethoprim/sulfamethoxazole, florfenicol, chloramphenicol, ciprofloxacin, colistin, and polymixin B. The bla NDM-5-carrying plasmids in 2023JX045 and 2024-406 were named p23045-NDM5 and p2024406-NDM5, respectively, with both belonging to the incompatibility (Inc)HI2/IncHI2A group and sequence type ST3. In 2023JX045, bla NDM-5 was flanked by two same-oriented copies of IS26 elements (IS26-dsbC-trpF-ble MBL-bla NDM-5-IS5-ΔIS3000-ΔISKox3-umuC-umuD-IS26). In 2024-406, p2024406-NDM5 was found to carry two copies of bla NDM-5, possibly resulting from duplication of IS26-dsbC-trpF-ble MBL-bla NDM-5-IS5-ΔISAba125-ΔIS3000-ΔISKox3-umuC-umuD-IS26 and interrupted by mobile element IS1 at the upstream region of ΔIS3000.

Discussion: This is the first report to describe the presence of two bla NDM-5 copies on an IncHI2/IncHI2A plasmid carried by serovar Stanley, as well as the dissemination of bla NDM-5 in Salmonella in Jiaxing City, China. IS26-flanked composite transposons appeared to play an important role in the formation of this region. The dissemination of blaNDM in Salmonella isolates and the complexity of the bla NDM-5 region highlight the urgent need to monitor carbapenem-resistant S. enterica.

Keywords: IS26 unit; IncHI2/IncHI2A; blaNDM–5; carbapenem-resistant; non-typhoidal Salmonella.

PubMed Disclaimer

Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

FIGURE 1
FIGURE 1
Schematic representation and comparison of the genetic environments of the blaNDM-flanking region in p23045-NDM5 and p2024406-NDM5 recovered from this study. Arrows indicate the direction of transcription of each gene. Numbers in brackets indicate nucleotide positions within corresponding plasmid sequences.
FIGURE 2
FIGURE 2
Phylogenetic tree of p23045-NDM5 and p2024406-NDM5 and 29 screened blaNDM-carrying plasmid of Salmonella isolates from GenBank.
See this image and copyright information in PMC

Similar articles

See all similar articles

References

    1. Alikhan N., Petty N., Ben Zakour N., Beatson S. (2011). BLAST Ring Image Generator (BRIG): Simple prokaryote genome comparisons. BMC Genomics 12:402. 10.1186/1471-2164-12-402 - DOI - PMC - PubMed
    1. Bathoorn E., Rossen J., Lokate M., Friedrich A., Hammerum A. (2015). Isolation of an NDM-5-producing ST16 Klebsiella pneumoniae from a Dutch patient without travel history abroad, August 2015. Euro Surveill. 20:30040. 10.2807/1560-7917.ES.2015.20.41.30040 - DOI - PubMed
    1. Chen Y., Zhou Z., Jiang Y., Yu Y. (2011). Emergence of NDM-1-producing Acinetobacter baumannii in China. J. Antimicrob Chemother. 66 1255–1259. 10.1093/jac/dkr082 - DOI - PubMed
    1. China National Center for Food Safety Risk Assessment (2024). National foodborne disease surveillance manual in 2024. Beijing: China National Center for Food Safety Risk Assessment.
    1. Day M., Meunier D., Doumith M., de Pinna E., Woodford N., Hopkins K. (2015). Carbapenemase-producing Salmonella enterica isolates in the UK. J. Antimicrob Chemother. 70 2165–2167. 10.1093/jac/dkv075 - DOI - PubMed

LinkOut - more resources