The mitochondrial genome of the firefly, Pyrocoelia amplissima (Olivier, 1886) (Coleoptera: Lampyridae) and its phylogenetic analysis

Abstract

We presents the first complete mitochondrial genome of Pyrocoelia amplissima (Olivier, 1886) (Coleoptera: Lampyridae: Pyrocoelia), a Chinese endemic firefly. The 17,426 bp circular genome contains 13 PCGs, 23 tRNAs, 2 rRNAs, and a control region with a notable A + T bias (76.91%). Phylogenetic analysis of 12 firefly species using 13 PCGs places P. amplissima basally within its genus. This complete mitochondrial genome provides valuable genetic information for further research on Lampyridae.

Keywords: Lampyridae; Pyrocoelia amplissima; mitochondrial genome; phylogeny.

Figure 1.

The specimen of Pyrocoelia amplissima (Olivier, 1886), (a) larval, scale bar = 10 mm. (b) adult, female on the left, male on the right, scale bar = 10 mm. Photographed and processed by Mao Wang.

Figure 2.

The circular-mapping mitochondrial genome of Pyrocoelia amplissima: the total length was 17,426 bp, which was divided into 37 genes, including 13 PCGs, 22 tRNAs, two rRNAs.

Figure 3.

The maximum likelihood (ML) tree of P. amplissima and twelve other firefly species based on the concatenated 13 PCGs. The NCBI accession numbers of the mitochondrial genomes used for tree construction are listed as follows: Diaphanes citrinus NC_051869 (Yang and Fu 2019)), Diaphanes nubilus NC_044787 (Chen et al. 2019), Diaphanes pectinealis NC_044793 (Chen et al. 2019), Pyrocoelia rufa AF452048 (Bae et al. 2004), Pyrocoelia analis OK323960 (Ji and Xu 2022), Pyrocoelia praetexta NC_044790 (Chen et al. 2019), Pyrocoelia thibetana NC_044792 (Chen et al. 2019), Vesta impressicollis MT554384, Abscondita terminalis NC_044776 (Chen et al. 2019), Abscondita anceyi NC_039706 (Hu and Fu 2018), Pristolycus diversecostatus NC_067973, Rhagophthalmus ohbai AB267275 (Li et al. 2007).