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Comparative Study
. 2025 Jul 11:15:04206.
doi: 10.7189/jogh.15.04206.

Differences in metabolic adaptations during mid and late pregnancy: a comparative cohort study between Rwanda and Germany

Alemayehu Amberbir #  1 , Madeleine Ordnung #  2 , Sage Marie Consolatrice Ishimwe  1 , Ronald Biemann  3 , Mandy Vogel  4   5   6 , Wieland Kiess  4   5   6 , Antje Körner  4   5   6 , Balkachew Nigatu  7 , Darius Bazimya  8 , Theogene Uwizeyimana  8 , Jean Baptiste Niyibizi  9 , Daniel Seifu  9 , Abebe Bekele  10 , Jon Genuneit  2   6
Affiliations
Comparative Study

Differences in metabolic adaptations during mid and late pregnancy: a comparative cohort study between Rwanda and Germany

Alemayehu Amberbir et al. J Glob Health. .

Abstract

Background: While cross-ancestral differences in glucose and lipid metabolism are widely reported in adults, there is a paucity of data on pregnant women during various stages of pregnancy. There is no consensus on what defines normal lipid ranges during pregnancy. Establishing reference ranges is crucial to reduce the risk of missing associated maternal and fetal health issues. Therefore, we aimed to investigate the metabolic profiles of healthy pregnant women and to establish national Rwandan reference ranges for these metabolites.

Methods: We derived the data from two ongoing longitudinal cohort studies conducted in predominantly rural Rwanda and urban Germany (Leipzig), providing repeat data from the second and third trimesters of pregnancy. We measured concentrations of glucose, total cholesterol (TC), high-density lipoprotein cholesterol (HDL), and triglycerides (TG), and estimated their associations with trimesters and cohorts using multivariable linear regression. We estimated the reference ranges using the 5th and 95th percentiles for each metabolic marker.

Results: For Rwanda and Leipzig, lipids and lipoproteins increased across trimesters, except for HDL, which remained equally low for Rwanda and significantly decreased for Leipzig. Concentrations of TC, low-density lipoprotein, and non-HDL were significantly higher in Leipzig compared to Rwanda for both trimesters, while HDL was significantly lower in Rwanda. Rwanda exhibited significantly higher TG levels in the second trimester than Leipzig, although this difference did not persist into the third trimester. Glucose concentrations were significantly higher in Rwanda than in Leipzig for both trimesters.

Conclusions: This is the first representative study investigating lipid and lipoprotein concentrations in pregnant women from Rwanda and comparing them to a European sample. This study shows that lipid, lipoprotein, and glucose concentrations differ by ancestry and stage of pregnancy. The higher TG and glucose concentrations in Rwanda may indicate an emerging burden of metabolic disorders in Africa.

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Conflict of interest statement

Disclosure of interest: The authors completed the ICMJE Disclosure of Interest Form (available upon request from the corresponding author) and disclose no relevant interests.

Figures

Figure 1
Figure 1
Distribution of the metabolic biomarkers during the second and third trimesters in Rwanda and Leipzig.
Figure 2
Figure 2
Percentile charts for the metabolic biomarkers.
See this image and copyright information in PMC

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