Capturing α-synuclein aggregation interactors using UltraID-LIPA

Abstract

Teixeira et al. present UltraID-light-inducible protein aggregation (UltraID-LIPA), a technique that combines optogenetic induction of α-synuclein aggregation with proximity-based proteomics. This system enables high-resolution capture of early aggregation events in live cells and implicates known and novel endolysosomal proteins, offering a robust framework for dissecting early pathogenic mechanisms in synucleinopathies and guiding future innovations.

Keywords: Parkinson’s disease; disease heterogeneity; protein interactome; protein misfolding; strain diversity; synucleinopathies.