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. 2025 Oct;312(4):1185-1194.
doi: 10.1007/s00404-025-08101-8. Epub 2025 Jul 12.

Structural rearrangements affect blastocyst development

Yizi Wang #  1   2   3 , Yuanlin Ma #  1   2   3 , Yanling Tan #  1   2   3 , Jing Wang  1   2   3 , Jiafu Pan  1   2   3 , Junli Song  1   2   3 , Yali Wang  1   2   3 , Yanwen Xu  4   5   6
Affiliations

Structural rearrangements affect blastocyst development

Yizi Wang et al. Arch Gynecol Obstet. 2025 Oct.

Abstract

Purpose: It is disputable whether chromosomal translocations lead to an inferior embryo development. The purpose of this study was to evaluate whether structural rearrangements (SR) affect blastocyst formation as compared to monogenic disorders in preimplantation genetic testing (PGT) cycles.

Methods: A total of 791 PGT-SR cycles and 757 PGT-M cycles from January 2021 to May 2023 were included.

Results: Lower blastocyst formation (graded 3BB or higher) rate was detected in the PGT-SR group compared with the control PGT-M group. In addition, lower proportion of day 5 blastocysts was found in the PGT-SR group compared with the control PGT-M group. Overall, a comparatively 12.7% lower proportion of eligible blastocysts in PGT-SR cycles. As expected, there were fewer balanced/normal blastocysts for transfer in the PGT-SR group (balanced/normal blastocysts rate, 32.3 vs. 59.9%, P = 0.02). The estimated curve by inverse model showed that yields of transferrable balanced/normal blastocyst per cycle came to a plateau stage followed with a rapid rise once the oocytes retrieved reached to the number of 20.4 in PGT-M cycle and 28.3 in PGT-SR cycle respectively.

Conclusions: Our results demonstrated that patients with SR had a high chance of obtaining lower blastocyst development and significantly fewer usable blastocysts available for transfer compared to PGT-M in their first ovarian stimulation cycle.

Keywords: Blastocyst development; Blastocyst formation; Preimplantation genetic testing; Structural rearrangements.

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Conflict of interest statement

Declarations. Conflict of interest: The authors declare that there is no conflict of interest. Ethical approval: Institutional review board approval was obtained for this study by the institutional review board of the First Affiliated Hospital of Sun Yet-Sen University [Application ID: (2018)029; Date of Approval: January 2018]. This research was conducted in accordance with the Helsinki Declaration. Informed consent was waived due to the retrospective analysis of anonymized data. Consent to participate: Informed consent was waived due to the retrospective analysis of anonymized data. Consent to publish: Not applicable.

Figures

Fig. 1
Fig. 1
Stacked column diagrams of eligible blastocysts in each period of age
Fig. 2
Fig. 2
Estimated curves by the logarithmic and the inverse models in regards to the yields of normal/balanced blastocysts per aspiration as the increase of oocytes retrieved
See this image and copyright information in PMC

References

    1. Nakano T, Ammae M, Satoh M, Mizuno S, Nakaoka Y, Morimoto Y (2022) Analysis of clinical outcomes and meiotic segregation modes following preimplantation genetic testing for structural rearrangements using aCGH/NGS in couples with balanced chromosome rearrangement. Reprod Med Biol 21(1):e12476 - PMC - PubMed
    1. Vasilevska M, Ivanovska E, Kubelka Sabit K, Sukarova-Angelovska E, Dimeska G (2013) The incidence and type of chromosomal translocations from prenatal diagnosis of 3800 patients in the republic of macedonia. Balkan J Med Genet 16(2):23–28 - PMC - PubMed
    1. Franssen MT, Musters AM, van der Veen F, Repping S, Leschot NJ, Bossuyt PM, Goddijn M, Korevaar JC (2011) Reproductive outcome after PGD in couples with recurrent miscarriage carrying a structural chromosome abnormality: a systematic review. Hum Reprod Update 17(4):467–475 - PubMed
    1. Di-Battista A, Favilla BP, Zamariolli M, Nunes N, Defelicibus A, Armelin-Correa L, da Silva IT, Reymond A, Moyses-Oliveira M, Melaragno MI (2023) Premature ovarian insufficiency is associated with global alterations in the regulatory landscape and gene expression in balanced X-autosome translocations. Epigenetics Chromatin 16(1):19 - PMC - PubMed
    1. Olszewska M, Wiland E, Huleyuk N, Fraczek M, Midro AT, Zastavna D, Kurpisz M (2019) Chromosome (re)positioning in spermatozoa of fathers and sons - carriers of reciprocal chromosome translocation (RCT). BMC Med Genom 12(1):30 - PMC - PubMed

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