Whole genome sequence of a virulent and multidrug resistant Staphylococcus aureus strain MD02 isolated from a diabetic foot ulcer in Uganda

Abstract

Objective: This study presents the whole-genome sequence of Staphylococcus aureus strain MD02, isolated from a diabetic foot ulcer at a tertiary hospital in southwestern Uganda. The objective was to characterize the genome to understand the isolate's resistance and virulence potential.

Data description: Genomic DNA of S. aureus strain MD02 was extracted and sequenced using the Illumina platform, generating high-quality paired-end reads with an average genome coverage of 32.6×. Quality control was performed with FastQC and reads were trimmed using Trimmomatic. De novo assembly was carried out using SPAdes, resulting in a draft genome of 2,815,980 bp assembled into 68 contigs with a GC content of 32.5%. Quality analysis of the genome revealed 98% completeness, and 2.48% contamination and the closest type strain to our isolate was Staphylococcus aureus (GCA_000330825.2) with an average nucleotide identity of 97.14%% and genome coverage of 89.38% confirming species level identity and close relatedness. Annotation using NCBI Prokaryotic Genome Annotation Pipeline identified 2,701 protein coding genes.

Keywords: Complete genome sequence of S. aureus; Diabetic foot ulcer; Staphylococcus aureus; Whole-genome sequencing; Wound bacterium.