Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2025 Jun;17(Suppl 2):S1908-S1910.
doi: 10.4103/jpbs.jpbs_186_25. Epub 2025 Jun 18.

Evaluation of Antimicrobial Efficacy of Different Irrigants Mixed with Silver Nanoparticles - An In Vivo Study

Ajay Chhabra  1 , Himani Mehra  1 , Priyanka Yadav  1 , K P Ramya  1 , Saravana B Prathap  1 , Mallika Sengupta  2 , Sona J Parvathy  1 , Vandana Chhabra  3
Affiliations

Evaluation of Antimicrobial Efficacy of Different Irrigants Mixed with Silver Nanoparticles - An In Vivo Study

Ajay Chhabra et al. J Pharm Bioallied Sci. 2025 Jun.

Abstract

Aim: To evaluate the antimicrobial efficacy of different irrigants combined with silver nanoparticles.

Materials and methods: Thirty-five patients were divided into seven groups (two control and five experimental), including Group 1: negative control (normal saline), Group 2: positive control (sodium hypochlorite), and Group 3 (sodium hypochlorite + AgNP), Group 4 (Twin Kleen), Group 5 (Twin Kleen + AgNP), Group 6 (Q Mix), and Group 7 (Q Mix + AgNP). Pre- and post-treatment bacterial samples were collected for analysis.

Results: Sodium hypochlorite combined with silver nanoparticles demonstrated the highest antimicrobial efficacy among the tested irrigants, with a significant difference confirmed by the Chi-square statistical analysis.

Conclusion: Within the limitations of the study, it can be concluded that sodium hypochlorite when mixed with the silver nanoparticles showed higher antimicrobial efficacy as compared to the other tested groups.

Keywords: Antimicrobial efficacy; Q Mix; Twin Kleen; root canal irrigants; silver nanoparticles; sodium hypochlorite.

PubMed Disclaimer

Conflict of interest statement

There are no conflicts of interest.

Figures

Figure 1
Figure 1
Colony-forming units grown on blood agar
See this image and copyright information in PMC

Similar articles

See all similar articles

References

    1. Sharma R, Panadan J, Sharma M, Tanweer S. Antimicrobial effectiveness of different root canal irrigants on viability of root canal flora. J Clin Diagn Res. 2023;17:37–41. doi: 10.7860/JCDR/2023/59877.17505.
    1. Boutsioukis C, Arias-Moliz MT. Present status and future directions - irrigants and irrigation methods. Int Endod J. 2022;55:588–612. doi: 10.1111/iej. 13739. - PMC - PubMed
    1. Dioguardi M, Gioia GD, Illuzzi G, Laneve E, Cocco A, Troiano G. Endodontic irrigants: Different methods to improve efficacy and related problems. Eur J Dent. 2018;12:459–66. doi: 10.4103/ejd.ejd_56_18. - PMC - PubMed
    1. Bhandi S, Mehta D, Mashyakhy M, Chohan H, Testarelli L, Thomas J, et al. Antimicrobial efficacy of silver nanoparticles as root canal irrigant's: A systematic review. J Clin Med. 2021;10:1152. doi: 10.3390/jcm10061152. - PMC - PubMed
    1. Wu D, Fan W, Kishen A, Gutmann JL, Fan B. Evaluation of the antibacterial efficacy of silver nanoparticles against Enterococcus faecalis biofilm. J Endod. 2014;40:285–90. doi: 10.1016/j.joen.2013.08.022. - PubMed

LinkOut - more resources