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. 1985 Dec;50(3):922-4.
doi: 10.1128/iai.50.3.922-924.1985.

Cloning of the cytotoxin-hemolysin gene of Vibrio vulnificus

A C Wright, J G Morris Jr, D R Maneval Jr, K Richardson, J B Kaper

Cloning of the cytotoxin-hemolysin gene of Vibrio vulnificus

A C Wright et al. Infect Immun. 1985 Dec.

Abstract

Genes encoding the cytotoxin-hemolysin of Vibrio vulnificus were cloned in Escherichia coli by using the lytic cloning vector, lambda 1059. Subcloning in plasmid pBR325 resulted in the isolation of a 3.2-kilobase DNA fragment containing the cytotoxin gene. By using this fragment as a DNA probe, homologous gene sequences were detected in all 54 V. vulnificus strains studied; homologous sequences were present in none of 96 isolates from 29 other bacterial species.

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