Correction: Reduction of the RNA binding protein TIA1 exacerbates neuroinflammation in tauopathy

Abstract

[This corrects the article DOI: 10.3389/fnins.2020.00285.].

Keywords: RNA binding protein; hippocampus; microglia; neurodegeneration; neuroinflammation; tauopathy.

Figure 4

Reduction of TIA1 increases expression of mRNAs associated with microglial phagocytosis and increases synapse loss in advanced tauopathy. (A) log2 fold change of C1qa expression in WT TIA1+/+ vs. P301S TIA1+/+, P301S TIA1+/−, and P301S TIA1−/− at 5 months (light gray) and 9 months (dark gray). n = 3 subjects/genotype. (B) log2 fold change of C3 expression in WT TIA1+/+ vs. P301S TIA1+/+, P301S TIA1+/−, and P301S TIA1−/− at 5 months (light gray) and 9 months (dark gray). n = 3 subjects/genotype. (C) log2 fold change of TREM2 expression in WT TIA1+/+ vs. P301S TIA1+/+, P301S TIA1+/−, and P301S TIA1−/− at 5 months (light gray) and 9 months (dark gray). n = 3 subjects/genotype. (D) High resolution (40×) confocal maximum projection images depicting Synaptophysin (SYP, red) in the apex of the dentate gyrus including the dorsal and ventral dentate granule cell layers and hilus in WT TIA1+/+ and P301S subjects that are TIA1+/+, TIA1+/−, and TIA1−/− at 5 months (top) and 9 months (bottom). Scale = 25 μm. (E) Average size of SYP particles in (D), 5 months (light gray) and 9 months (dark gray). n = 9–12 fields, from 3 to 4 animals/genotype. (F) Percent Area occupied by SYP particles in (D), 5 months (light gray) and 9 months (dark gray). n = 9–12 fields, from 3 to 4 animals/genotype. (G) Electron photomicrographs at 20k × depicting synapses (Presynaptic structures in red, B, bouton; Ax, axon; Postsynaptic structures in blue, D, dendrite; Sp, spine; Synaptic contacts = white arrowheads) in the dentate gyrus hilus neuropil in WT TIA1+/+ and P301S subjects that are TIA1+/+, TIA1+/−, and TIA1−/− animals. Scale = 1 μm. (H) Average density of excitatory (light gray) and inhibitory (dark gray) synapses in the dentate gyrus hilus neuropil in WT TIA1+/+ and P301S TIA1+/+, TIA1+/−, and TIA1−/− animals. (n = 3–4 fields, from 1 animal/genotype, no statistical analyses performed, qualitative data). All statistical comparisons calculated with One-Way ANOVA with Bonferroni post hoc test, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.