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. 2025 Jul 7;104(10):105547.
doi: 10.1016/j.psj.2025.105547. Online ahead of print.

Effect of dietary Bacillus subtilis, dried whey powder, and Saccharomyces cerevisiae on the production performance, egg quality, blood biochemical parameters, and ileal histomorphology in late-phase Lohmann LSL- lite laying hens

Zahra Hamzehee  1 , Mehran Torki  2 , Khodabakhsh Rashidi  3 , Alireza Abdolmohammadi  1
Affiliations

Effect of dietary Bacillus subtilis, dried whey powder, and Saccharomyces cerevisiae on the production performance, egg quality, blood biochemical parameters, and ileal histomorphology in late-phase Lohmann LSL- lite laying hens

Zahra Hamzehee et al. Poult Sci. .

Abstract

The current study was conducted to investigate the effects of dietary whey powder (WP), Bacillus subtilis (B. subtilis), and Saccharomyces cerevisiae (S. cerevisiae) and their combination on the production performance, egg quality, blood biochemical and ileum histomorphology parameters of Lohmann laying hens. A total of 288 Lohmann LSL-Lite laying hens (75 wk) were randomly allocated to 8 treatments with 6 replicates of 6 hens each, over a 12-week period. A 2 × 2 × 2 factorial arrangement was used, consisting of a basal corn-soybean meal diet with two levels of B. subtilis (0 and 1 g/kg diet, 109 CFU), two levels of WP (0 and 1 g/kg diet), and two levels of S. cerevisiae (0 and 3 g/kg diet, 5 × 109 CFU). The control group received a standard diet without any additive. No significant three-way interaction among WP, B. subtilis, and S. cerevisiae was observed for the studied parameters, except for malondialdehyde level (P < 0.05), where a significant three-way interaction was detected, resulting in reduced malondialdehyde concentrations (P = 0.028). The simultaneous inclusion of WP and B. subtilis improved ileum villus length and serum calcium levels. A significant two-way interaction between WP and B. subtilis indicated a synergistic effect on egg production, weight, and mass during 75-80 weeks (P < 0.05). In conclusion, it seems that the simultaneous use of WP and B. subtilis is effective in improving performance and ileum histomorphology in Lohmann laying hens in the late production phase.

Keywords: Bacillus subtilis; Prebiotic; Probiotic; Saccharomyces cerevisiae; Whey powder.

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Conflict of interest statement

Disclosures The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Fig 1
Fig. 1
Photomicrographs of ileal tissues from different treatment groups: T1 (control), T2 (B. subtilis), T3 (S. cerevisiae), T4 (whey powder), T5 (whey powder + S. cerevisiae), T6 (B. subtilis + whey powder), T7 (B. subtilis + S. cerevisiae), and T8 (B. subtilis + whey powder + S. cerevisiae). VH = villus height; VW = villus width; CD = crypt depth. (H&E staining method).
See this image and copyright information in PMC

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