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. 2025 Jul 1;53(4):31-38.
doi: 10.15586/aei.v53i4.1348. eCollection 2025.

IFN-γ cocultured mesenchymal stem cells promote substantial immunomodulatory effects in mice models of allergic asthma

Tao Chen  1 , Jiaojiao Hu  1 , Yanping Shi  1 , Xia Li  1 , Yujun Wang  1 , Seyyed Shamsadin Athari  2 , Entezar Mehrabi Nasab  3   4 , Jingning Guo  5
Affiliations

IFN-γ cocultured mesenchymal stem cells promote substantial immunomodulatory effects in mice models of allergic asthma

Tao Chen et al. Allergol Immunopathol (Madr). .

Abstract

Asthma is a debilitating lung disease characterized by airway inflammation and airflow obstruction. Immune cells, particularly T helper 2 (Th2) lymphocytes, are central players in the pathogenesis of asthma and mesenchymal stem cells (MSCs) have shown the capability of softening pathological inflammatory responses in asthma. Hence, we researched the immunopathologic effects MSCs cocultured with interferon (IFN)-γ, the main Th1 cytokine, in asthmatic mice. After isolation, MSCs were cocultured with IFN-γ and administered to asthmatic mice. Subsequent analyses included enumeration of broncho-alveolar lavage (BAL) fluid's inflammatory cells, determination of the levels of immunoglobulin E (IgE), leukotrienes (LTs), cytokines, chemokines, and histopathology assessment. The administration of IFN-γ-cocultured MSCs reduced the percentage of eosinophils in the BAL fluid and levels of IgE, LTs, cytokines, and chemokines. Also, there was a decrease in the eosinophilic infiltration of perivascular areas and periairways. IFN-γ cocultured MSCs could modulate immune responses and harness pathological events in allergic asthma.

Keywords: allergy; biomedicine; immune response; lung; stem cell.

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Conflict of interest statement

There is no conflicts of interest.

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