Effects of adenine nucleotides and phosphate on adenosine triphosphate sulphurylase from Anabaena cylindrica
- PMID: 406902
- PMCID: PMC1164770
- DOI: 10.1042/bj1640161
Effects of adenine nucleotides and phosphate on adenosine triphosphate sulphurylase from Anabaena cylindrica
Abstract
Production of adenosine 5'-[35S]sulphatophosphate by a partially purified ATP sulphurylase from Anabaena cylindrica was inhibited by AMP, ADP and P1. Decreases in enzyme activity in the presence of these inhibitors were reversed by increasing the concentrations of ATP. The adenine nucleotides inhibited the enzyme competitively with respect to ATP. In the presence of P1, ATP showed a positive co-operative effect on enzyme activity. The inhibition by P1 was enhanced by increasing concentrations of MG2+. The effects of the adenine nucleotides and the interaction of P1 and Mg2+ on ATP sulphurylase activity are discussed in relation to the regulation of sulphate assimilation via the energy metabolism of the alga.
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