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. 2025 Jul 22;38(5):132.
doi: 10.1007/s13577-025-01263-2.

SMU-pRMS: a novel cell line of pleomorphic rhabdomyosarcoma

Naoya Nakahashi  1 , Makoto Emori  2 , Yasutaka Murahashi  1 , Kazuyuki Murase  3 , Junya Shimizu  1 , Kenji Murata  4 , Tomohide Tsukahara  4 , Shintaro Sugita  5 , Kousuke Iba  6 , Kohichi Takada  3 , Makoto Osanai  4 , Atsushi Teramoto  1
Affiliations

SMU-pRMS: a novel cell line of pleomorphic rhabdomyosarcoma

Naoya Nakahashi et al. Hum Cell. .

Abstract

Pleomorphic rhabdomyosarcoma (pRMS) is a rare and highly malignant mesenchymal tumor. Complete resection is the only curative treatment available, owing to the limited efficacy of chemotherapy and radiotherapy. Therefore, developing novel therapies for pRMS is important for improving clinical outcomes. Herein, a novel pRMS cell line, SMU-pRMS, was established for a detailed understanding of the biological characteristics of pRMS, thereby developing new therapies. A tissue sample from a surgically resected tumor of a 69-year-old patient was subjected to primary culture. The cell line was established and authenticated by evaluating the short tandem repeats of DNA microsatellites. Monolayer cultures of SMU-pRMS cells exhibited constant growth, spheroid formation, and invasiveness. These cells exhibited high chemosensitivity to eribulin. In addition, mice inoculated with SMU-pRMS cells developed tumors after 4 weeks. Therefore, the SMU-pRMS cell line is a useful tool for investigating pRMS development and evaluating novel therapeutic agents.

Keywords: Cell line; DNA microsatellite; Eribulin; Pleomorphic rhabdomyosarcoma; Tumor.

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Conflict of interest statement

Declarations. Conflict of interest: The authors declare that they either have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. Ethical approval: This study was approved by the Institutional Review Board of Sapporo Medical University (reference number 282–156). Written informed consent was obtained from the patient in accordance with the guidelines of the Declaration of Helsinki.

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