doi: 10.1016/0165-7992(85)90065-x.
Mutations induced by X-rays at the HPRT locus in cultured Chinese hamster cells are mostly large deletions
- PMID: 4069145
- DOI: 10.1016/0165-7992(85)90065-x
Item in Clipboard
Mutations induced by X-rays at the HPRT locus in cultured Chinese hamster cells are mostly large deletions
Mutat Res.
1985 Dec.
Abstract
We investigated the molecular basis of 19 X-ray-induced HPRT-deficient mutants of V79 Chinese hamster cells with Southern hybridisation techniques. 12 of those mutants suffer from a big deletion (greater than 10 kb) of HPRT DNA sequences. Cytological studies of chromosome preparations of those 12 deletion mutants showed that in at least 3 of these mutants part of the long arm of the X-chromosome was lost. After correction for spontaneous arising mutations we estimate that at least 70-80% of X-ray-induced mutations are caused by large deletions.
Similar articles
-
Molecular analysis of X-ray-induced mutants at the HPRT locus in V79 Chinese hamster cells.Int J Radiat Biol Relat Stud Phys Chem Med. 1986 Jun;49(6):1011-20. doi: 10.1080/09553008514553231. Int J Radiat Biol Relat Stud Phys Chem Med. 1986. PMID: 3486844
-
Mutation screening of bleomycin-induced V79 Chinese hamster hprt mutants using multiplex polymerase chain reaction.Mutagenesis. 1991 Nov;6(6):527-31. doi: 10.1093/mutage/6.6.527. Mutagenesis. 1991. PMID: 1724791
-
Molecular characterization of 125I decay and X-ray-induced HPRT mutants in CHO cells.Int J Radiat Biol Relat Stud Phys Chem Med. 1987 Feb;51(2):193-9. doi: 10.1080/09553008714550691. Int J Radiat Biol Relat Stud Phys Chem Med. 1987. PMID: 2881903
-
Ionizing radiation and genetic risks. III. Nature of spontaneous and radiation-induced mutations in mammalian in vitro systems and mechanisms of induction of mutations by radiation.Mutat Res. 1991 Jul;258(1):75-97. doi: 10.1016/0165-1110(91)90029-u. Mutat Res. 1991. PMID: 2023601 Review.
-
The molecular nature of mutations in cultured mammalian cells: a review.Mutat Res. 1985 Jun-Jul;150(1-2):431-42. doi: 10.1016/0027-5107(85)90140-x. Mutat Res. 1985. PMID: 3889619 Review. No abstract available.
Cited by
-
Ionizing radiation-induced mutagenesis.Br J Cancer. 1988 Jan;57(1):6-18. doi: 10.1038/bjc.1988.2. Br J Cancer. 1988. PMID: 3279995 Free PMC article. Review. No abstract available.
-
Mutator phenotype of Werner syndrome is characterized by extensive deletions.Proc Natl Acad Sci U S A. 1989 Aug;86(15):5893-7. doi: 10.1073/pnas.86.15.5893. Proc Natl Acad Sci U S A. 1989. PMID: 2762303 Free PMC article.
-
Hypomutability in Fanconi anemia cells is associated with increased deletion frequency at the HPRT locus.Proc Natl Acad Sci U S A. 1990 Nov;87(21):8383-7. doi: 10.1073/pnas.87.21.8383. Proc Natl Acad Sci U S A. 1990. PMID: 2236046 Free PMC article.
-
The original shaker-with-syndactylism mutation (sy) is a contiguous gene deletion syndrome.Mamm Genome. 1998 Nov;9(11):889-92. doi: 10.1007/s003359900889. Mamm Genome. 1998. PMID: 9799839 Free PMC article.
-
High frequency of genomic deletions induced by Me-lex, a sequence selective N3-adenine methylating agent, at the Hprt locus in Chinese hamster ovary cells.Mutat Res. 2009 Dec 1;671(1-2):58-66. doi: 10.1016/j.mrfmmm.2009.08.013. Epub 2009 Sep 1. Mutat Res. 2009. PMID: 19729030 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous
