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. 2025 Aug 14;14(8):e0099222.
doi: 10.1128/mra.00992-22. Epub 2025 Jul 22.

Hybrid reference genome assembly for the archetypal Leishmania (Viannia) guyanensis bearing the dsRNA totivirus LRV1

Peter J Myler #  1   2   3 , Gowthaman Ramasamy  1 , Wesley C Warren #  4 , Deborah E Dobson  5 , Stephen M Beverley #  5
Affiliations

Hybrid reference genome assembly for the archetypal Leishmania (Viannia) guyanensis bearing the dsRNA totivirus LRV1

Peter J Myler et al. Microbiol Resour Announc. .

Abstract

We report a high-quality hybrid genome assembly and annotation for a Leishmania (Viannia) guyanensis line (M4147) expressing a firefly luciferase reporter gene and bearing the totivirus LRV1 strongly implicated in parasite hypervirulence. This reference genome should facilitate the study of infectivity and pathogenesis of cutaneous and severe leishmaniasis.

Keywords: Leishmania guyanensis; South America; Trypanosomatidae; Viannia; comparative genomics; kinetoplastida; mucocutaneous leishmaniasis.

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Conflict of interest statement

The authors declare no conflict of interest.

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