Introduction to the CLEM technique developed in the field of neuroanatomy

Abstract

CLEM, which allows the observation of the same structure using both light and electron microscopes, is becoming increasingly popular in various fields in recent years as molecular and cell biology research deepens and experimental techniques become more sophisticated. Although CLEM is often considered a specialized technique, it has been used for 40 years to observe neuroanatomy since the era of Golgi-EM, so it does not require specialized equipment and can be applied relatively easily to various tissues and even cultured cells at a single cell level. The specific methodology is introduced here.

Keywords: Confocal laser scanning microscopy; Immunoelectron microscopy; Multiple immunofluorescence staining; PAP method; Rapid freezing and thawing method.