Nanofabrication Techniques for Enhancing Plant-Microbe Interactions in Sustainable Agriculture

Abstract

Nanomaterials have emerged as a transformative technology in agricultural science, offering innovative solutions to improve plant-microbe interactions and crop productivity. The unique properties, such as high surface area, tunability, and reactivity, of nanomaterials, including nanoparticles, carbon-based materials, and electrospun fibers, render them ideal for applications such as nutrient delivery systems, microbial inoculants, and environmental monitoring. This review explores various types of nanomaterials employed in agriculture, focusing on their role in enhancing microbial colonization and soil health and optimizing plant growth. Key nanofabrication techniques, including top-down and bottom-up manufacturing, electrospinning, and nanoparticle synthesis, are discussed in relation to controlled release systems and microbial inoculants. Additionally, the influence of surface properties such as charge, porosity, and hydrophobicity on microbial adhesion and colonization is examined. Moreover, the potential of nanocoatings and electrospun fibers to enhance seed protection and promote beneficial microbial interactions is investigated. Furthermore, the integration of nanosensors for detecting pH, reactive oxygen species, and metabolites offers real-time insights into the biochemical dynamics of plant-microbe systems, applicable to precision farming. Finally, the environmental and safety considerations regarding the use of nanomaterials, including biodegradability, nanotoxicity, and regulatory concerns, are addressed. This review emphasizes the potential of nanomaterials to revolutionize sustainable agricultural practices by improving crop health, nutrient efficiency, and environmental resilience.

Keywords: electrospinning; microbial inoculants; nanocoatings; nanomaterials; nanosensors; plant-microbe interactions; surface properties; sustainable agriculture.

Figure 1

Various modern approaches for synthesizing nanoparticles.

Figure 2

Comparison between green and chemical synthesis methods for synthesizing nanoparticles.

Figure 3

Synthesis application modes and benefits of nanofertilizers and nanopesticides.

Figure 4

Illustration of the nanostructured seed coatings and their interactions with microorganisms to facilitate microbial adhesion and promote seedling growth.

Figure 5

Applications of biosensors for monitoring microbial colonization.

Figure 6

Schematic of an LOC device for profiling root exudates and detecting secondary metabolites.

Figure 7

Possible nanotoxicity in the soil ecosystem.