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Comparative Study
. 1977 Jun;13(6):389-97.
doi: 10.1007/BF02615099.

Fish cell culture characteristics of a cell line from the silver perch Bairdiella chrysura

Comparative Study

Fish cell culture characteristics of a cell line from the silver perch Bairdiella chrysura

J H Wharton et al. In Vitro. 1977 Jun.

Abstract

A cell designated SP-1 was established from tissue of the silver perch, Bairdiella chrysura. Cells were fibroblast-like and grew best at 26 degrees C in Leibovitz medium (L-15) containing 15% fetal bovine serum and 0.150 M sodium chloride. Passage 1 to passage 9 SP-1 cells contained a chromosome number of 48; at passages 27 and 50 the modal numbers were 51 and 54, respectively. Confirmation of the origin of SP-1 cells was made by the cytotoxic antibody dye-exclusion test. This cell line supported the growth of lymphocystis virus from the silver perch but was not found to replicate various other fish and mammalian viruses.

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