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. 2025 Jul 25;16(1):1408.
doi: 10.1007/s12672-025-03252-1.

Notch1 and CyclinD1 expression and significance in jaw ameloblastoma: an immunohistochemical analysis

Baoyinbatu Gabiyatu #  1   2 , Wenchao Li #  2 , Rihan Wu  3 , Yuan Tian  2 , Senhao Li  2 , Sarnai Tsagaankhuu  1 , Li-Dao Bao  2 , Damdindorj Boldbaatar  4
Affiliations

Notch1 and CyclinD1 expression and significance in jaw ameloblastoma: an immunohistochemical analysis

Baoyinbatu Gabiyatu et al. Discov Oncol. .

Abstract

Purpose: Ameloblastoma (AM), a frequently encountered odontogenic epithelial neoplasm, demonstrates complex molecular pathogenesis involving dysregulation of cellular signaling networks. This study investigated the clinicopathological significance of Notch1 signaling pathway activation and its downstream effector CyclinD1 in jaw ameloblastoma progression.

Methods: Immunohistochemical profiling was conducted on 66 archived AM specimens and 23 normal oral mucosa (NOM) controls. Protein localization patterns and expression intensities were systematically analyzed using chi-square tests, Fisher's exact test, and Spearman's rank correlation (significance threshold α = 0.05).

Results: A notable proportion of jaw AM samples exhibited marked immunoreactivity for Notch1 and CyclinD1 proteins, predominantly localized in the cellular membrane and cytoplasm. Significantly elevated levels of Notch1 and CyclinD1 proteins were evident in AM compared to NOM (P < 0.05). The protein expression levels showed no association with AM subtype (P > 0.05), while a positive correlation between Notch1 and CyclinD1 expression was noted in AM (r = 0.509, P < 0.05).

Conclusions: The coordinated overexpression of Notch1-CyclinD1 axis in ameloblastoma pathogenesis suggests their synergistic involvement in tumor proliferation dynamics. These findings position the Notch signaling pathway as a potential therapeutic target for AM management strategies.

Keywords: Ameloblastoma; CyclinD1; Notch1; Proliferation.

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Conflict of interest statement

Declarations. Ethics approval and consent to participate: All participants have provided informed consent by signing the requisite documentation.This study has received approval from the Ethics Committee of Mongolia National Medical University (2023/3–04). All procedures performed in this study involving human participants were in accordance with the Declaration of Helsinki (as revised in 2013). Competing interests: The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
Histopathology. A AM (×100), B UA (×100), C PA (×100), D MA (×100)
Fig. 2
Fig. 2
Immunohistochemical characteristics in NOM. A CyclinD1 (×200), B Notch1 (×200)
Fig. 3
Fig. 3
Immunohistochemical characteristics of Notch1. A CA (×400), B UA (×400), C EA (×400), D MA (×400)
Fig. 4
Fig. 4
Immunohistochemical characteristics of CyclinD1. A CA (×400), B UA (×400), C EA (×400), D MA (×400)
See this image and copyright information in PMC

References

    1. Kreppel M, Zöller J. Ameloblastoma-Clinical, radiological, and therapeutic findings. Oral Dis. 2018;24(1–2):63–6. 10.1111/odi.12702. - PubMed
    1. Effiom OA, Ogundana OM, Akinshipo AO, Akintoye SO. Ameloblastoma: current etiopathological concepts and management. Oral Dis. 2018;24(3):307–16. 10.1111/odi.12646. - PubMed
    1. Soluk-Tekkesin M, Wright JM. The world health organization classification of odontogenic lesions: A summary of the changes of the 2022. Ed Turk Patoloji Derg. 2022;38(2):168–84. 10.5146/tjpath.2022. (5th). - PMC - PubMed
    1. Salehinejad J, Zare-Mahmoodabadi R, Saghafi S, Jafarian AH, Ghazi N, Rajaei AR, et al. Immunohistochemical detection of p53 and PCNA in ameloblastoma and adenomatoid odontogenic tumor. J Oral Sci. 2011;53(2):213–7. 10.2334/josnusd.53.213. - PubMed
    1. Sandra F, Mitsuyasu T, Nakamura N, Shiratsuchi Y, Ohishi M. Immunohistochemical evaluation of PCNA and Ki-67 in ameloblastoma. Oral Oncol. 2001;37(2):193–8. 10.1016/s1368-8375(00)00079-8. - PubMed

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