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. 2025 Jul 28;192(8):530.
doi: 10.1007/s00604-025-07361-0.

Identification of cariogenic bacteria by click chemistry mediated polyethylene glycolized graphyne nanozymes

Lelin Qian #  1 , Zhangli Yu #  1 , Yuan Zhang  1 , Jingyi Huang  1 , Daixin Ye  2 , Lili Niu  3 , Juan Zhang  4
Affiliations

Identification of cariogenic bacteria by click chemistry mediated polyethylene glycolized graphyne nanozymes

Lelin Qian et al. Mikrochim Acta. .

Abstract

Dental caries, one of the most common oral diseases, is mainly induced by multiple cariogenic bacteria in the oral microenvironment, so it is important to construct a method that can identify oral multiply cariogenic bacteria. Herein, a machine learning assisted colorimetric sensing array has been constructed by using polyethylene glycolized graphyne nanozymes, which have been firstly prepared through click chemistry to enhance the peroxidase-like catalytic activity and stability. Taking advantage of different interactions between cariogenic bacteria and polyethylene glycolized graphyne nanozymes, the sensing array can well identify ten oral cariogenic bacteria and their binary and ternary complexes by linear discriminant analysis algorithm. In view of its high accuracy to analyze the spiked artificial saliva samples, the developed colorimetric sensing array owns a great potential for the diagnosis of dental caries.

Keywords: Dental caries; Cariogenic bacteria; Machine learning; Polyethylene glycolized graphyne nanozymes; Sensing arrays.

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Conflict of interest statement

Declarations. Competing interests: The authors declare no competing interests.

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References

    1. Peres MA, Macpherson LMD, Weyant RJ, Daly B, Venturelli R, Mathur MR, Listl S, Celeste RK, Guarnizo-Herreño CC, Kearns C, Benzian H, Allison P, Watt RG (2019) Oral diseases: a global public health challenge. The Lancet 394(10194):249–260
    1. Watt RG, Daly B, Allison P, Macpherson LMD, Venturelli R, Listl S, Weyant RJ, Mathur MR, Guarnizo-Herreño CC, Celeste RK, Peres MA, Kearns C, Benzian H (2019) Ending the neglect of global oral health: time for radical action. The Lancet 394(10194):261–272
    1. Pitts NB, Zero DT, Marsh PD, Ekstrand K, Weintraub JA, Ramos-Gomez F, Tagami J, Twetman S, Tsakos G, Ismail A (2017) Dental caries Nature Reviews Disease Primers 3:17030 - PubMed
    1. Saraithong P, Pattanaporn K, Chen Z, Khongkhunthian S, Laohapensang P, Chhun N, Pattanaporn W, Gaw HY, Li Y (2015) Streptococcus mutans and Streptococcus sobrinus colonization and caries experience in 3- and 5-year-old Thai children. Clin Oral Invest 19(8):1955–1964
    1. Dame-Teixeira N, Parolo CC, Maltz M, Tugnait A, Devine D, Do T (2016) Actinomyces Spp. gene expression in root caries lesions. J Oral Microbiol 8:32383

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