Chinese expert consensus on hematologic malignancy karyotyping

Zailin Yang¹, Shuang Chen¹, Mingyu Zhao¹, Fei Gao², Jianwei Li³, Yu Peng¹, Tingting Jiang¹, Jun Zhang⁴, Xinyi Tang⁵, Yakun Zhang⁵, Fan Chen⁶, Hongwei Chen⁷, Pu Chen⁸, Siyu Chen⁹, Xingqin Huang¹⁰, Yujiao Jia¹¹, Xiaohong Xie¹², Chengwen Li¹¹, Guoxia Li¹³, Jieping Li¹, Zhaoquan Li¹⁴, Zheng Li¹⁵, Rong Liang¹⁶, Jie Liu¹⁷, Ling Liu¹⁸, Fang Long¹⁹, Zhigao Long²⁰, Jinglong Lü¹², Qiang Ma²¹, Ping Mao²², Bin Pan²³, Jinlan Pan²⁴, Ling Pan²⁵, Xiangui Peng⁹, Xing Qiang⁹, Longrong Ran¹, Fanggang Ren²⁶, Xiuhua Sun²⁷, Dangfeng Wang²⁸, Li Wang²⁹, Shiyi Wang³⁰, Xingzhe Wang³¹, Yanfang Wang³², Ying Wang³³, Zhiyuan Wang²³, Xia Wei³⁴, Zhiwei Wu³⁵, Menghua Wu⁶, Xuelian Wu¹, Jianwen Xiao³⁶, Junjun Yang³⁷, Zesong Yang¹⁴, Yuanyou Yao¹⁰, Yi Ying²², Yalan Yu³⁸, Meiling Zhang³⁹, Ping Zhang⁴⁰, Wei Zhang⁴¹, Hui Zhou⁴², Jianfeng Zhou⁶, Mingxia Zhu³², Liqun Zou⁴³, Cui Mao⁴⁴, Ximing Yang⁴⁵, Yao Liu⁴⁶

Affiliations

¹ Department of Hematology-Oncology, Chongqing University Cancer Hospital, Chongqing 400030, China.
² Department of Cytogenetics Laboratory, Sichuan Kingmed Center for Clinical Laboratory CO., LTD, Chengdu 610052, China.
³ Department of Cytogenetics Laboratory, Tianjin Kingmed Center for Clinical Laboratory CO., LTD, Tianjin 300392, China.
⁴ Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China.
⁵ Department of Hematology-Oncology, Chongqing University Cancer Hospital, Chongqing 400030, China; School of Medicine, Chongqing University, Chongqing 400044, China.
⁶ Department of Cytogenetics Laboratory, Guangzhou Kingmed Center for Clinical Laboratory CO., LTD, Guangzhou 510000, China.
⁷ Department of Clinical Laboratory, The First Hospital of Qinhuangdao, Qinhuangdao 066000, China.
⁸ Department of Laboratory Medicine, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
⁹ Medical Center of Hematology, Xinqiao Hospital of Third Military Medical University (Army Medical University), Chongqing 400037, China.
¹⁰ Department of Hematology, Southwest Hospital of Third Military Medical University (Army Medical University), Chongqing 400038, China.
¹¹ Department of Pathology and Laboratory Medicine, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.
¹² Department of Hematology, Chongqing University Three Gorges Hospital, Chongqing 404100, China.
¹³ Institute of Hematology, The Second Hospital of Shanxi Medical University, Taiyuan 030001, China.
¹⁴ Department of Hematology, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
¹⁵ Department of Hematology Laboratory, Army Specialty Medical Center Army Medical University, Chongqing 400042, China.
¹⁶ Department of Hematology, Xijing Hospital, Air Force Medical University, Xi'an 710032, China.
¹⁷ Department of Clinical Laboratory, The Fifth Clinical Medical College of Shanxi Medical University (Shanxi Provincial People's Hospital), Taiyuan 030000, China.
¹⁸ Department of Prevention and Healthcare, Southwest Hospital of Third Military Medical University (Army Medical University), Chongqing 400038, China.
¹⁹ Department of Clinical Laboratory, Sichuan Provincial Maternity and Child Health Care Hospital, Chengdu 610000, China.
²⁰ Center for Medical Genetics, School of Life Sciences, Central South University, Changsha 410078, China.
²¹ Department of Clinical Laboratory, the Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, China.
²² Department of Hematology, Guangzhou First People's Hospital, Guangzhou 510180, China.
²³ Department of Hematology, The Affiliated Hospital of Xuzhou Medical University, XuZhou 221002, China.
²⁴ Department of Hematology, The First Affiliated Hospital of Suzhou University, Suzhou 215000, China.
²⁵ Department of Hematology, Sichuan University West China Hospital, Chengdu 610041, China.
²⁶ Department of Hematology Laboratory, The Second Hospital of Shanxi Medical University, Taiyuan 030001, China.
²⁷ Department of Lymphoma and Head-Neck Oncology, The Second Hospital of Dalian Medical University, Dalian 116027, China.
²⁸ Department of Laboratory of Cytogenetics, Beijing Qinghe Hospital, Beijing 100912, China.
²⁹ Shanghai Institute of Hematology, State Key Laboratory of Medical Genomics, National Research Center for Translational Medicine at Shanghai, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
³⁰ Department of Clinical Laboratory, Children's Hospital of Chongqing Medical University, Chongqing 400014, China.
³¹ Department of Key Laboratory of Hematology, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, China.
³² Department of Hematology, Peking University Third Hospital, Beijing 100191, China.
³³ Hematology Laboratory of Tongji Hospital of Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China.
³⁴ Department of Hematology, The Third Affiliated Hospital of Chongqing Medical University, Chongqing 401120, China.
³⁵ Department of Clinical Laboratory, The First Affiliated Hospital of USTC, Hefei 230001, China.
³⁶ Department of Hematology-Oncology, Children Hospital of Chongqing, Chongqing 400016, China.
³⁷ Department of Hematology, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou 325000, China.
³⁸ Department of Hematology, Zhongnan Hospital of Wuhan University, Wuhan 430071, China.
³⁹ Zhengzhou Kingmed Center for Clinical Laboratory CO., LTD, Zhengzhou 450016, China.
⁴⁰ Department of Hematology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China.
⁴¹ Department of Hematology, Peking Union Medical College Hospital, Beijing 100730, China.
⁴² Hunan Cancer Hospital and Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha 410013, China.
⁴³ Department of Cancer Medicine, Cancer Center, West China Hospital of Sichuan University, Chengdu 610041, China.
⁴⁴ Department of Cytogenetics Laboratory, Tianjin Kingmed Center for Clinical Laboratory CO., LTD, Tianjin 300392, China. Electronic address: zb-maocui@kingmed.com.cn.
⁴⁵ Department of Clinical laboratory, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China. Electronic address: yximing2005@163.com.
⁴⁶ Department of Hematology-Oncology, Chongqing University Cancer Hospital, Chongqing 400030, China. Electronic address: liuyao77@cqu.edu.cn.

PMID: 40721192
DOI: 10.1016/j.cca.2025.120507

Review

Chinese expert consensus on hematologic malignancy karyotyping

Zailin Yang et al. Clin Chim Acta. 2026.

. 2026 Jan 1:578:120507.

doi: 10.1016/j.cca.2025.120507. Epub 2025 Jul 26.

Authors

Affiliations

¹ Department of Hematology-Oncology, Chongqing University Cancer Hospital, Chongqing 400030, China.
² Department of Cytogenetics Laboratory, Sichuan Kingmed Center for Clinical Laboratory CO., LTD, Chengdu 610052, China.
³ Department of Cytogenetics Laboratory, Tianjin Kingmed Center for Clinical Laboratory CO., LTD, Tianjin 300392, China.
⁴ Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China.
⁵ Department of Hematology-Oncology, Chongqing University Cancer Hospital, Chongqing 400030, China; School of Medicine, Chongqing University, Chongqing 400044, China.
⁶ Department of Cytogenetics Laboratory, Guangzhou Kingmed Center for Clinical Laboratory CO., LTD, Guangzhou 510000, China.
⁷ Department of Clinical Laboratory, The First Hospital of Qinhuangdao, Qinhuangdao 066000, China.
⁸ Department of Laboratory Medicine, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
⁹ Medical Center of Hematology, Xinqiao Hospital of Third Military Medical University (Army Medical University), Chongqing 400037, China.
¹⁰ Department of Hematology, Southwest Hospital of Third Military Medical University (Army Medical University), Chongqing 400038, China.
¹¹ Department of Pathology and Laboratory Medicine, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.
¹² Department of Hematology, Chongqing University Three Gorges Hospital, Chongqing 404100, China.
¹³ Institute of Hematology, The Second Hospital of Shanxi Medical University, Taiyuan 030001, China.
¹⁴ Department of Hematology, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
¹⁵ Department of Hematology Laboratory, Army Specialty Medical Center Army Medical University, Chongqing 400042, China.
¹⁶ Department of Hematology, Xijing Hospital, Air Force Medical University, Xi'an 710032, China.
¹⁷ Department of Clinical Laboratory, The Fifth Clinical Medical College of Shanxi Medical University (Shanxi Provincial People's Hospital), Taiyuan 030000, China.
¹⁸ Department of Prevention and Healthcare, Southwest Hospital of Third Military Medical University (Army Medical University), Chongqing 400038, China.
¹⁹ Department of Clinical Laboratory, Sichuan Provincial Maternity and Child Health Care Hospital, Chengdu 610000, China.
²⁰ Center for Medical Genetics, School of Life Sciences, Central South University, Changsha 410078, China.
²¹ Department of Clinical Laboratory, the Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, China.
²² Department of Hematology, Guangzhou First People's Hospital, Guangzhou 510180, China.
²³ Department of Hematology, The Affiliated Hospital of Xuzhou Medical University, XuZhou 221002, China.
²⁴ Department of Hematology, The First Affiliated Hospital of Suzhou University, Suzhou 215000, China.
²⁵ Department of Hematology, Sichuan University West China Hospital, Chengdu 610041, China.
²⁶ Department of Hematology Laboratory, The Second Hospital of Shanxi Medical University, Taiyuan 030001, China.
²⁷ Department of Lymphoma and Head-Neck Oncology, The Second Hospital of Dalian Medical University, Dalian 116027, China.
²⁸ Department of Laboratory of Cytogenetics, Beijing Qinghe Hospital, Beijing 100912, China.
²⁹ Shanghai Institute of Hematology, State Key Laboratory of Medical Genomics, National Research Center for Translational Medicine at Shanghai, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
³⁰ Department of Clinical Laboratory, Children's Hospital of Chongqing Medical University, Chongqing 400014, China.
³¹ Department of Key Laboratory of Hematology, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, China.
³² Department of Hematology, Peking University Third Hospital, Beijing 100191, China.
³³ Hematology Laboratory of Tongji Hospital of Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China.
³⁴ Department of Hematology, The Third Affiliated Hospital of Chongqing Medical University, Chongqing 401120, China.
³⁵ Department of Clinical Laboratory, The First Affiliated Hospital of USTC, Hefei 230001, China.
³⁶ Department of Hematology-Oncology, Children Hospital of Chongqing, Chongqing 400016, China.
³⁷ Department of Hematology, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou 325000, China.
³⁸ Department of Hematology, Zhongnan Hospital of Wuhan University, Wuhan 430071, China.
³⁹ Zhengzhou Kingmed Center for Clinical Laboratory CO., LTD, Zhengzhou 450016, China.
⁴⁰ Department of Hematology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China.
⁴¹ Department of Hematology, Peking Union Medical College Hospital, Beijing 100730, China.
⁴² Hunan Cancer Hospital and Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha 410013, China.
⁴³ Department of Cancer Medicine, Cancer Center, West China Hospital of Sichuan University, Chengdu 610041, China.
⁴⁴ Department of Cytogenetics Laboratory, Tianjin Kingmed Center for Clinical Laboratory CO., LTD, Tianjin 300392, China. Electronic address: zb-maocui@kingmed.com.cn.
⁴⁵ Department of Clinical laboratory, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China. Electronic address: yximing2005@163.com.
⁴⁶ Department of Hematology-Oncology, Chongqing University Cancer Hospital, Chongqing 400030, China. Electronic address: liuyao77@cqu.edu.cn.

PMID: 40721192
DOI: 10.1016/j.cca.2025.120507

Abstract

Chromosomal karyotype analysis is crucial for diagnosing and managing hematologic malignancies, yet inconsistent protocols compromise reliability. To standardize practices, this consensus integrates international guidelines (WHO, ICC, ELN) with clinical expertise, providing evidence-based recommendations for specimen collection, cell culture, chromosome harvesting, banding techniques, karyotype analysis, and reporting. By establishing uniform operational standards, this initiative seeks to mitigate variability, improve detection rates, and support precision medicine in hematologic malignancies.

Keywords: Chromosome; Chromosome banding; Cytogenetics; Hematologic malignancies; Karyotype analysis.

PubMed Disclaimer

Conflict of interest statement

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Publication types

Actions

MeSH terms

Actions
Actions
Actions
Actions
Actions
Actions
Actions

Supplementary concepts

Actions

LinkOut - more resources

Full Text Sources
- Elsevier Science

Save citation to file

Email citation

Add to Collections

Add to My Bibliography

Your saved search

Create a file for external citation management software

Your RSS Feed

Chinese expert consensus on hematologic malignancy karyotyping

Affiliations

Chinese expert consensus on hematologic malignancy karyotyping

Authors

Affiliations

Abstract

Conflict of interest statement

Publication types

MeSH terms

Supplementary concepts

LinkOut - more resources

Full Text Sources