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. 2025 Jul 28;25(1):288.
doi: 10.1186/s12906-025-05027-1.

Acute and subacute toxicity of the aqueous extract of Cissus Polyantha Glig and Bradt (Vitaceae) on biochemical and hematological parameters in Wistar rats

David Miaffo  1 , Oulianovie Guessom Kamgue  2 , Kilenma Kolefer  3 , Elizé Dadaya  4 , Talba Abba Mahamad  5 , Barthelemy Maidadi  5 , Albert Kamanyi  6
Affiliations

Acute and subacute toxicity of the aqueous extract of Cissus Polyantha Glig and Bradt (Vitaceae) on biochemical and hematological parameters in Wistar rats

David Miaffo et al. BMC Complement Med Ther. .

Abstract

Background: Cissus polyantha (C. polyantha) Glig and Bradt is a climbing plant of the Vitaceae family generally distributed in tropical regions and used in traditional African pharmacopoeia for the treatment of conjunctivitis, pain, inflammation, microbial diseases and diabetes mellitus. The aim of this study was to evaluate the acute and subacute toxicity of the aqueous extract from leafy stems of C. polyantha (AECP) in rats.

Methods: In acute toxicity, AECP was administered orally at a single dose of 2000 mg/kg. Clinical signs, general behavior and mortality were assessed for 14 days. In subacute toxicity, AECP at 111, 222 and 444 mg/kg was administered orally for 28 days. Body weight, internal organ weight, water intake, food consumption, biochemical parameters, hematological profile and histological examinations were evaluated.

Results: Acute toxicity results showed no signs of poisoning and no mortality. The mean lethal dose (LD50) of AECP was therefore greater than 2000 mg/kg. In subacute toxicity, a significant increase in body weight, water and food consumption, liver weight and lymphocyte counts as well as a decrease in ALT activity and creatinine levels were recorded in rats of both sexes treated at 222 and/or 444 mg/kg. AECP caused a notable reduction in uric acid, total cholesterol and triglycerides at all doses tested. Histopathological analysis of rat kidneys shows slight mesengial hyperplasia at 444 mg/kg. However, all other parameters evaluated did not experience significant variation after AECP administration at all doses.

Conclusions: In acute toxicity, the LD50 is greater than 2000 mg/kg, so AECP is of low toxicity. In subacute toxicity, only the dose of 444 mg/kg of AECP resulted in decreased body weight, water and food consumption, and increased liver weight, ALT activity, and lymphocyte counts. Doses below 444 mg/kg may therefore be safe.

Keywords: Cissus Polyantha; Acute toxicity; Aqueous extract; Biochemical parameters; Haematological profile; Histology; Subacute toxicity; Wistar rats.

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Conflict of interest statement

Declarations. Ethics approval and consent to participate: All animal experiments were conducted in accordance with the Cameroon National Ethics Committee (ref. N° FWIRB 00001954) and approved by the Bioethics Advisory Commission of the Faculty of Sciences, University of Maroua (ref: No14/0261/Uma/D/FS/VD-RC). Informed consent was also sought from all study participants prior to inclusion in the study. Consent to publish: Not applicable. Competing interests: The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
: Effects of AECP on relative body weight, food intake and water consumption of male and female animals. Data were presented as mean ± SEM (n = 10). *p < 0.05; **p < 0.01 difference compared to the control group. Relative body weight in male (A1), relative body weight in female (A2), food intake in male (B1), food intake in female (B2), water intake in male (C1), water intake in female (C2), Aqueous extract from leafy stems of C. polyantha (AECP)
Fig. 2
Fig. 2
: Effects of AECP on the liver sections in male (A) and female (B) rats (H-E, 400 X). a = Control group; b = 111 mg/kg; c = 222 mg/kg; d = 444 mg/kg. Hepatocyte (H), hepatic artery (HA), Portal vein (PV), Bile duct (BD), Sinusoid (S), Aqueous extract from leafy stems of C. polyantha (AECP)
Fig. 3
Fig. 3
: Effects of AECP on the kidney sections in male (A) and female (B) rats (H-E, 400 X). a = Control group; b = 111 mg/kg; c = 222 mg/kg; d = 444 mg/kg. Glomerulus (G), Proximal tubule (PT), Urinary space (Us), Distal tubule (DT), Aqueous extract from leafy stems of C. polyantha (AECP)
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