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. 1977 Jul;6(1):58-61.
doi: 10.1128/jcm.6.1.58-61.1977.

Serum antibody to Pseudomonas aeruginosa exotoxin measured by a passive hemagglutination assay

M Pollack, N S Taylor

Serum antibody to Pseudomonas aeruginosa exotoxin measured by a passive hemagglutination assay

M Pollack et al. J Clin Microbiol. 1977 Jul.

Abstract

A passive hemagglutination (PHA) assay for antibody to Pseudomonas aeruginosa exotoxin is described which utilizes chromic chloride-treated ovine erythrocytes coated with purified toxin. PHA antitoxin titers correlated well with those obtained by a cytotoxicity neutralization assay (r = 0.91, P less than 0.001), whereas the PHA assay was four to eight times as sensitive. The mean serum PHA titer of 16 patients convalescing from recent pseudomonas infections (log2 = 9.4 +/- 3.9) was significantly higher (P less than 0.001) than that of 17 healthy controls (2,7 +/- 2.6), and fourfold or greater rises were demonstrated in 5 of 7 patients examined sequentially. The lower levels of PHA antibody in sera from 11 of 17 controls suggested the acquisition of antitoxin secondary to asymptomatic infection.

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References

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