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. 2025 Jul;123(2):e70318.
doi: 10.1111/tpj.70318.

Dynamic m6A RNA methylation correlates with ethylene-induced petal senescence and may modulate antioxidant and metabolic pathways in carnation (Dianthus caryophyllus L.)

Hongya Wang #  1   2   3   4 , Chuansen Zhao #  1   2   3   4 , Zekang Pan  1   2   3   4 , Xilin Li  1   2   3   4 , Siqi Wang  1   2   3   4 , Xinyi Yuan  1   2   3   4 , Chunlin Zhu  1   2   3   4 , Zheng Sun  1   2   3   4 , Min Wang  1   2   3   4 , Shan Feng  1   2   3   4 , Zeqing Li  5 , Linlin Zhong  1   2   3   4 , Yunjiang Cheng  1   2   3   4 , Manzhu Bao  1   4   6   7 , Fan Zhang  1   2   3   4   6   7   8   9
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Dynamic m6A RNA methylation correlates with ethylene-induced petal senescence and may modulate antioxidant and metabolic pathways in carnation (Dianthus caryophyllus L.)

Hongya Wang et al. Plant J. 2025 Jul.

Abstract

Petal senescence is a critical stage in the life cycle of plants and involves a complex regulatory network, in which ethylene is one of the important regulatory factors. As a key RNA modification, the role of m6A methylation in various physiological processes in plants has been widely recognized, but its specific function in petal senescence has not been thoroughly investigated. In this study, LC-MS/MS analysis showed that ethylene treatment significantly reduced the level of m6A in carnation (Dianthus caryophyllus L.) petals. MeRIP-seq analysis further showed that ethylene treatment resulted in a change in the distribution pattern of m6A modification, especially an increase in enrichment near the stop codon region. Further studies identified four aging-related genes, including antioxidant genes (DcGST10 and DcGPX8) and TCA cycle genes (DcACLA and DcACLB). The m6A levels of these genes were positively correlated with their mRNA expression levels. Furthermore, we found that silencing these genes significantly accelerates carnation petal senescence and increases oxidative stress, which highlights the key role of m6A modification in modulating antioxidant and metabolic pathways. This study confirms the dynamic changes and potential mechanisms of m6A modification during carnation petal senescence, providing new insights into the epigenetic regulatory network during plant senescence.

Keywords: ATP‐citrate lyase; carnation; ethylene; m6A; oxidative stress; petal senescence.

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