Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2025 Jul 9;10(28):30002-30012.
doi: 10.1021/acsomega.4c10512. eCollection 2025 Jul 22.

Acid Phosphatase Produced by in Solid Fermentation Using Millet

Jussara Maria Martins de Almeida Afonso  1 , Frederico Alves Lima  1 , Miriam Maria de Resende  1
Affiliations

Acid Phosphatase Produced by in Solid Fermentation Using Millet

Jussara Maria Martins de Almeida Afonso et al. ACS Omega. .

Abstract

The absorption of organic phosphorus from the environment and its efficient use requires the action of a large class of enzymes called acid phosphatases. Fungi can convert insoluble phosphorus into soluble forms during the acidification and chelation stages or through hydrolysis mediated by phosphatase enzymes. These enzymes are obtained from plants, animal cells, and microorganisms. Acid phosphatases (AcPases) have their optimum pH of action being less than 6.00. This study evaluated the production and extraction of acid phosphatases using solid fermentation (FES) with supported in millet. Preliminary tests were fundamental in analyzing the enzyme production potential of the strain, where the best results were for millet. Using a Central Composite Design (CCD), it was possible to develop equations for responses to acid phosphatase activity (U/mL) and dry biomass (g/L) using the variables: millet mass, inoculum concentration, and moisture. The best results were for solid fermentation, using the millet substrate with cultivation in sterile distilled water and extraction with Tween 80, where there was a growth of biomass and enzymatic activity, whose optimized final values were 9.27 ± 0.53 g/L and 36.09 ± 0.61 U/mL, respectively, after 9 days of solid fermentation.

PubMed Disclaimer

Figures

1
1
Morphology of the mycelium of T. harzianum growing in PDA (CK) medium by scanning electron microscopy.
2
2
(a) Response surface and (b) contour plot for acid phosphatase activity with moisture (%) and millet mass (g).
3
3
(a) Response surface and (b) contour plot for acid phosphatase activity in relation to inoculum concentration (g/L) and millet mass (g).
4
4
(a) Response surface and (b) contour plot for acid phosphatase activity as a function of inoculum concentration (g/L) and moisture (%).
5
5
(a) Response surface and (b) contour plot for dry biomass with moisture (%) and millet mass (g).
6
6
(a) Response surface and (b) contour plot for dry biomass with inoculum (g/L) and millet mass (g).
7
7
(a) Response surface and (b) contour plot for dry biomass with inoculum (g/L) and moisture (%).
8
8
Desirability function profiles for simultaneous maximization of acid phosphatase activity (AcPase) and biomass concentration responses.
See this image and copyright information in PMC

References

    1. Bouras H., Mamassi A., Devkota K. P., Choukr-Allah R., Bouazzama B.. Integrated effect of saline water irrigation and phosphorus fertilization practices on wheat (Triticum aestivum) growth, productivity, nutrient content and soil proprieties under dryland farming. Plant Stress. 2023;10:100295. doi: 10.1016/j.stress.2023.100295. - DOI
    1. Zhang J., Feng L., Ouyang Y., Hu R., Xu H., Wang J.. Phosphate-solubilizing bacteria and fungi in relation to phosphorus availability under different land uses for some latosols from Guangdong, China. Catena. 2020;195:104686. doi: 10.1016/j.catena.2020.104686. - DOI
    1. Zaman U., Khan S. U., Hendi A. A., Rehman K., Badshah S., Refat M. S., Alsuhaibani A. M., Ullah K. K., Wahab A.. Kinetic and thermodynamic studies of novel acid phosphatase isolated and purified from Carthamus oxyacantha seedlings. Int. J. Biol. Macromol. 2023;224:20–31. doi: 10.1016/j.ijbiomac.2022.12.025. - DOI - PubMed
    1. Chu Y. H., Yu X. X., Jin X., Wang Y. T., Zhao D. J., Zhang P., Sun G. M., Zhang Y. H.. Purification and characterization of alkaline phosphatase from lactic acid bacteria. RSC Adv. 2019;9:354–360. doi: 10.1039/C8RA08921C. - DOI - PMC - PubMed
    1. da Silva G., Ramos L. F. C., dos Santos Seckler H. F., Gomes J. M., Cortines I. R., Ramos C., Anobom E. D., Machado D. M. A., Oliveira P.. Biochemical characterization of digestive membrane associated alkaline phosphatase from the velvet bean caterpillar Anticarsia gemmatalis . Arch. Insect Biochem. Physiol. 2019;102:e21591. doi: 10.1002/arch.21591. - DOI - PubMed

LinkOut - more resources