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. 2025 Jul 29;170(8):187.
doi: 10.1007/s00705-025-06373-8.

Biological characterization and genomic profiling of a novel Providencia phage isolated from farm effluents

Mantong Zhu #  1   2 , Chang Liu #  2 , Guangfeng Liu  2 , Hongsai Zhang  2 , Xing Wang  2 , Ying Wang  2 , Yongqi Luo  2 , Jingzhe Jiang  3
Affiliations

Biological characterization and genomic profiling of a novel Providencia phage isolated from farm effluents

Mantong Zhu et al. Arch Virol. .

Abstract

A Providencia strain was isolated from farm effluents and used as a host to isolate a bacteriophage named A06. A double-layer agar plaque assay was used to determine the optimal multiplicity of infection (MOI), construct a one-step growth curve, evaluate the temperature and pH stability of the phage, and assess its in vitro antibacterial activity and lysis efficiency. Whole-genome sequencing, functional annotation, and comparative genomics analysis were used to analyze its biological features and determine its taxonomic position. Bacteriophage A06 has a circular DNA genome with 58 open reading frames (ORFs). The optimal MOI was determined to be 100, and a one-step growth curve showed the latent period to be 40 minutes and the lysis period to range from 40 to 90 minutes. Bacteriophage A06 exhibited a high degree of thermal stability and pH tolerance, and genomic analysis indicated that it represents a new species within the genus Kakivirus of the family Autoscriptoviridae.

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Conflict of interest statement

Declarations. Competing interests: The authors declare no competing interests.

References

    1. Cornaglia G, Frugoni S, Mazzariol A et al (1995) Activities of oral antibiotics on Providencia strains isolated from institutionalized elderly patients with urinary tract infections. Antimicrob Agents Chemother 39:2819–2821. https://doi.org/10.1128/AAC.39.12.2819 - DOI - PubMed - PMC
    1. Dealler SF, Hawkey PM, Millar MR (1988) Enzymatic degradation of urinary indoxyl sulfate by Providencia stuartii and Klebsiella pneumoniae causes the purple urine bag syndrome. J Clin Microbiol 26:2152–2156. https://doi.org/10.1128/jcm.26.10.2152-2156.1988 - DOI - PubMed - PMC
    1. Haynes J, Hawkey PM (1989) Providencia alcalifaciens and travellers’ diarrhoea. BMJ 299:94–95. https://doi.org/10.1136/bmj.299.6691.94-a - DOI - PubMed - PMC
    1. Yoh M, Matsuyama J, Ohnishi M et al (2005) Importance of Providencia species as a major cause of travellers’ diarrhoea. J Med Microbiol 54:1077–1082. https://doi.org/10.1099/jmm.0.45846-0 - DOI - PubMed
    1. Wenzel RP, Hunting KJ, Osterman CA, Sande MA (1976) Providencia stuartii, a hospital pathogen: potential factors for its emergence and transmission. Am J Epidemiol 104:170–180. https://doi.org/10.1093/oxfordjournals.aje.a112287 - DOI - PubMed

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